| 棉铃虫被中华卵索线虫感染后血淋巴游离脂肪酸的含量变化 |
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| 引用本文： | 王茂先,邢树文,丁有雄,王国秀.棉铃虫被中华卵索线虫感染后血淋巴游离脂肪酸的含量变化[J].韩山师范学院学报,2005,26(6):15-18. |
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| 作者姓名： | 王茂先 邢树文 丁有雄 王国秀 |
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| 作者单位： | 1. 韩山师范学院生物系,广东,潮州,521041
2. 华中师范大学,生命科学学院,武汉,430079 |
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| 基金项目： | 国家自然科学基金项目（30170129）. |
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| 摘 要： | 用气相色谱法（GC）分别对中华卵索线虫感染棉铃虫不同时期血淋巴中游离脂肪酸含量变化进行了分析．棉铃虫血淋巴的软脂酸、棕榈油酸、硬脂酸、油酸、亚油酸、亚麻酸等的含量都比其它类型脂肪酸的含量高得多；感染和未感染棉铃虫血淋巴中不饱和脂肪酸的相对百分含量均在80％左右，感染1～3d，感染组血淋巴中的不饱和脂肪酸总量逐渐下降，饱和脂肪酸情况相反；4d后，不饱和脂肪酸总量逐渐上升，而饱和脂肪酸情况又相反．上述研究结果将为研制接近于宿主血淋巴成分的体外培养液，促进中华卵索线虫体外培养获得成功提供了一定的理论依据．
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| 关 键 词： | 中华卵索线虫 棉铃虫 血淋巴 游离脂肪酸 |
| 文章编号： | 1007-6883（2005）06-0015-04 |
| 收稿时间： | 2004-11-29 |
| 修稿时间： | 2004-11-29 |
Change in the Contents of Free Fatty Acids in Haemolymph of Heliothis armigera Infected by Ovomermis sinensis (Nematode) |
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| WANG Mao-xian,XING Shu-wen,DING You-xiong,WANG Guo-xiu.Change in the Contents of Free Fatty Acids in Haemolymph of Heliothis armigera Infected by Ovomermis sinensis (Nematode)[J].Journal of Hanshan Teachers College,2005,26(6):15-18. |
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| Authors: | WANG Mao-xian XING Shu-wen DING You-xiong WANG Guo-xiu |
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| Institution: | 1.Department of Biology, Hanshan Teachers College, Chaozhou 521041,China 2.College of Life Sciences, Central China Normal University, Wuhan 430079,China |
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| Abstract: | The insect parasitic nematode, Ovomermis sinensis Chen et al., 1991 has the potential for the biological control of a range of insect pests due to many insect species susceptible to this nematode, for example Leucania separata,Heliothis armigera,prodenia litura,Spodoptera exigua, Plutella xylostella,Pieris rapae,Agrotis ypsilon.Euxoa segetum etc. The insect host invariably dies when the juvenile nematode completes its parasitic development and exits from the host's hemocoel. There is an important significance for studying the biochemical component in the haemolymph of one of its host, cotton bollworm Heliothis armigera so as to offer the indispensable biochemical parameter to succeeding in confecting the culture medium. The effects on the contents of free fatty acids in the haemolymph of Heliothis armigera infected by Ovomermis sinensis were studied. The relative levels of other free fatty acids were much lower and varied completely. The relative levels (%) of unsaturated fatty acids in the infected and uninfected Heliothis armigera were all 80% or so. During the first three days of infection, the levels of unsaturated fatty acids in the haemolymph of Heliothis armigera of the infection set declined little by little. But for the saturated fatty acids, it was on the contrary. After four days of infection, the level of unsaturated fatty acids rose little by little, and the saturated fatty acids were again on the contrary. The relative levels of Palmitic acid, Palmitoleic acid, Stearic acid, Oleic acid, Linoleic acid and Linolenic acid etc in the haemolymph of Heliothis armigera were much higher than that of other fatty acids, compared with the control group. In the facet of the researching and confection on the culture medium of Ovomermis sinensis, the level and constitutes of the culture medium should be taken into account the above research result. These several kinds of free fatty acids, including Palmitic acid, Palmitoleic acid, Stearic acid, Oleic acid, Linoleic acid and Linolenic acid etc have high and other free fatty acids are indispensable. Moreover, the content of free fatty acids in the culture medium is adjusted in time according to the parasitic stages of Ovomermis sinensis. Those research results have the referenced value to succeeding in cultivation of the entomogenous nematode Ovomermis sinensis in vitro. |
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| Keywords: | Heliothis armigera Ovomermis sinensis haemolymph free fatty acid |
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