Development of a large-scale isolation chamber system for the safe and humane care of medium-sized laboratory animals harboring infectious diseases |
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| Authors: | Xin Pan Jian-cheng Qi Ming Long Hao Liang Xiao Chen Han Li Guang-bo Li and Hao Zheng |
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| Institution: | Department of Microbiology, the Second Military Medical University, Shanghai 200433, China |
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| Abstract: | The close phylogenetic relationship between humans and non-human primates makes non-human primates an irreplaceable model
for the study of human infectious diseases. In this study, we describe the development of a large-scale automatic multi-functional
isolation chamber for use with medium-sized laboratory animals carrying infectious diseases. The isolation chamber, including
the transfer chain, disinfection chain, negative air pressure isolation system, animal welfare system, and the automated system,
is designed to meet all biological safety standards. To create an internal chamber environment that is completely isolated
from the exterior, variable frequency drive blowers are used in the air-intake and air-exhaust system, precisely controlling
the filtered air flow and providing an air-barrier protection. A double door transfer port is used to transfer material between
the interior of the isolation chamber and the outside. A peracetic acid sterilizer and its associated pipeline allow for complete
disinfection of the isolation chamber. All of the isolation chamber parameters can be automatically controlled by a programmable
computerized menu, allowing for work with different animals in different-sized cages depending on the research project. The
large-scale multi-functional isolation chamber provides a useful and safe system for working with infectious medium-sized
laboratory animals in high-level bio-safety laboratories. |
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| Keywords: | Multi-function Medium-sized animal Isolation chamber Biological safety |
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