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| 马疯树硬酯酰-酰基载体蛋白脱饱和酶基因的克隆及特征 | |
| 引用本文： | 罗通,马丹炜,徐莺,邓骛远,肖猛,卿人韦,陈放.马疯树硬酯酰-酰基载体蛋白脱饱和酶基因的克隆及特征[J].上海大学学报(英文版),2007,11(2):182-188. |
| 作者姓名： | 罗通 马丹炜 徐莺 邓骛远 肖猛 卿人韦 陈放 |
| 作者单位： | College of Life Sciences Sichuan University，College of Life Sciences，Sichuan University，College of Life Sciences，Sichuan University，Department of Bioengineering，Yibin University，College of Life Sciences，Sichuan University，College of Life Sciences，Sichuan University，College of Life Sciences，Sichuan University，Chengdu 610064，P.R.China Department of Bioengineering，Yibin University，Yibin 644007，P.R.China，Chengdu 610064，P.R.china，Chengdu 610064，P.R.china，Yibin 644007，P.R.China，Chengdu 610064，P.R.China，Chengdu 610064，P.R.China，Chengdu 610064，P.R.China |
| 基金项目： | Project supported by ＂Tenth Five Years＂ Key Program of the State Science and Technology Commission in China （Grant Nos.2002BA901A15, 2004BA411B01） |
| 摘 要： | Using degenerate primers and RT-PCR,RACE techniques,a 1491 bp cDNA segment of stearoyl-acyl carrier protein desaturase(SAD)is cloned from developing seeds of Jatropha curcas L.The segment contains a 1191 bp of complete open reading frame(ORF).Analysis in the BLAST on NCB! shows that Jatropha curcas SAD(JSAD)gene encodes a protein precursor composed of a signal peptide of 33 amino acids and a mature peptide of 363 amino acids.The homological analysis shows that JSAD has high level of homology both in nucleotide sequence and in amino acid sequence to other plants SADs.The nucleotide and peptide identity of JSAD to Ricinus communis SAD(RSAD)is up to 89% and 96.2% respectively.Molecular modeling of JSAD indicates that its three-dimensional structure strongly resembled the crystal structure of RSAD. |
| 关 键 词： | 麻疯树 硬脂酰基-载体蛋白脱饱和酶 脂肪酸 基因克隆 生物信息分析 |
| 收稿时间： | 2005-08-24 |
| 修稿时间： | 2005-12-13 |
Cloning and characterization of a stearoyl-ACP desaturase gene from Jatropha curcas |
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| LUO Tong,MA Dan-wei,XU Ying,DENG Wu-yuan,XIAO Meng,QING Ren-wei,CHEN Fang.Cloning and characterization of a stearoyl-ACP desaturase gene from Jatropha curcas[J].Journal of Shanghai University(English Edition),2007,11(2):182-188. | |
| Authors: | LUO Tong MA Dan-wei XU Ying DENG Wu-yuan XIAO Meng QING Ren-wei CHEN Fang |
| Institution: | 1. College of Life Sciences, Sichuan University, Chengdu 610064, P. R. China;Department of Bioengineering, Yibin University, Yibin 64t007, P. R. China 2. College of Life Sciences, Sichuan University, Chengdu 610064, P. R. China 3. Department of Bioengineering, Yibin University, Yibin 64t007, P. R. China |
| Abstract: | Using degenerate primers and RT-PCR,RACE techniques,a 1491 bp cDNA segment of stearoyl-acyl carrier protein desaturase(SAD)is cloned from developing seeds of Jatropha curcas L.The segment contains a 1191 bp of complete open reading frame(ORF).Analysis in the BLAST on NCB! shows that Jatropha curcas SAD(JSAD)gene encodes a protein precursor composed of a signal peptide of 33 amino acids and a mature peptide of 363 amino acids.The homological analysis shows that JSAD has high level of homology both in nucleotide sequence and in amino acid sequence to other plants SADs.The nucleotide and peptide identity of JSAD to Ricinus communis SAD(RSAD)is up to 89% and 96.2% respectively.Molecular modeling of JSAD indicates that its three-dimensional structure strongly resembled the crystal structure of RSAD. |
| Keywords: | Jatropha curcas stearoyl-acyl carrier protein desaturase fatty acid gene cloning bioinformatics analysis |
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