This paper described the idea of construction of Siemens automation lab based on S7-300PLC. It changes the traditional experimental mode based on single PLC. PLC control technology and industry communication are combined through PROFIBUS and PROFINET. Physical controlled object is also equipped in this lab to provide a practice platform for students to simulate the control technology of real industry automation process. The experiments are introduced according to the lecture. It is proved that the system can combine flexibly to realize needed configuration to satisfy different lecture's requirements.