超高效液相色谱法测定广西蜜梨花芽中4种植物激素 |
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引用本文: | 黄岛平,徐慧,蒋艳芳.超高效液相色谱法测定广西蜜梨花芽中4种植物激素[J].大众科技,2011(10):184-185,195. |
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作者姓名: | 黄岛平 徐慧 蒋艳芳 |
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作者单位: | 广西分析测试研究中心,广西南宁,530022 |
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基金项目: | 广西自治区直属公益性科研院所基本科研业务专项基金资助项目(2009ACZ10) |
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摘 要: | 采用超高效液相色谱,C18色谱柱,PDA紫外检测器,以甲醇-0.1%冰乙酸水溶液为流动相,流速0.3mL/min,检测波长为260nm的条件下。同时测定吲哚乙酸、脱落酸赤霉素玉米素4种激素,各峰的分离效果理想,10min内完成了测定。玉米素平均回收率为98.2%,RSD为3.6%,赤霉素平均回收率为98.2%,RSD为2.6%,吲哚乙酸平均回收率为98.8%,RSD为2.8%,脱落酸平均回收率为101.1%,RSD为3.3%。
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关 键 词: | 超高效液相色谱 蜜梨花芽 植物激素 |
Determination of Four Endogenous Hormones in Honey Floral Bud of Guangxi by UPLC |
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Abstract: | A method was created for the determination of indole-3-acid (IAA), abscisic-acid (ABA), gibberllin (GA3), zeatin (ZT) in Guangxi by using a C18 chromatographic column and a UV detector (λ =260nm). The mobile plase was composed of methanol and 0.1% acetica acid and the flow rate was 0.3% mL/min. These hormones were separated well in i0 min with the average recoveries of ZT, IAA, ABA, GA3 were 98.2%, 98.8%, 101. 1%, and 98.2% while the RSDs of them were 3.6%,2.8%,3.3%,2.6%. |
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Keywords: | UHPLC Honey Floral Bud Endogenous Hormones |
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