Membrane-integrated microfluidic device for high-resolution live cell imaging |
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| Authors: | Epshteyn Alla A Maher Steven Taylor Amy J Holton Angela B Borenstein Jeffrey T Cuiffi Joseph D |
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| Institution: | 1Bioengineering Center, Charles Stark Draper Laboratory, Tampa, Florida 33612, USA;2College of Public Health, University of South Florida, Tampa, Florida 33612, USA;3Charles Stark Draper Laboratory, Department of Bioengineering, Cambridge, Massachusetts 02139, USA |
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| Abstract: | The design and fabrication of a membrane-integrated microfluidic cell culture device (five layers,≤500?μm total thickness) developed for high resolution microscopy is reported here. The multi-layer device was constructed to enable membrane separated cell culture for tissue mimetic in vitro model applications and pharmacodynamic evaluation studies. The microdevice was developed via a unique combination of low profile fluidic interconnect design, substrate transfer methodology, and wet silane bonding. To demonstrate the unique high resolution imaging capability of this device, we used oil immersion microscopy to image stained nuclei and mitochondria in primary hepatocytes adhered to the incorporated membrane |
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