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Dynamic in situ chromosome immobilisation and DNA extraction using localized poly(N-isopropylacrylamide) phase transition |
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| Authors: | Eriksen Johan Thilsted Anil Haraksingh Marie Rodolphe Lüscher Christopher James Nielsen Lars Bue Svendsen Winnie Edith Szabo Peter Kristensen Anders |
| Institution: | 1DTU Nanotech, Department of Micro- and Nanotechnology, Technical University of Denmark, Building 345b, Ørsteds Plads, DK-2800 Kongens Lyngby, Denmark;2DTU Chemical Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Building 227, Søltofts Plads, DK-2800 Kongens Lyngby, Denmark |
| Abstract: | A method of in situ chromosome immobilisation and DNA extraction in a microfluidic polymer chip was presented. Light-induced local heating was used to induce poly(N-isopropylacrylamide) phase transition in order to create a hydrogel and embed a single chromosome such that it was immobilised. This was achieved with the use of a near-infrared laser focused on an absorption layer integrated in the polymer chip in close proximity to the microchannel. It was possible to proceed to DNA extraction while holding on the chromosome at an arbitrary location by introducing protease K into the microchannel. |
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