| 拟穴青蟹抗菌肽scygonadin在毕赤酵母中的表达及其抗菌活性 |
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| 引用本文： | 许婉芳,谢嘉华,陈慧芸.拟穴青蟹抗菌肽scygonadin在毕赤酵母中的表达及其抗菌活性[J].泉州师范学院学报,2011,29(6):11-15. |
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| 作者姓名： | 许婉芳 谢嘉华 陈慧芸 |
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| 作者单位： | 1. 泉州师范学院化学与生命科学学院,福建泉州,362000
2. 厦门大学,海洋与环境学院,近海海洋环境科学国家重点实验室,福建厦门361005 |
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| 基金项目： | 泉州市科技计划项目(2010Z55); 服务海西重点建设项目(A101) |
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| 摘 要： | 采用PCR方法克隆获得拟穴青蟹抗菌肽scygonadin基因成熟肽片段，将其连接到Pichia pastoris酵母表达载体pPIC9K中，构建了分泌表达载体pPICgk-Scy．电击转化毕赤酵母GS115，经表型筛选和PCR鉴定证实了目的基因已稳定整合人酵母基因组中．以甲醇诱导表达阳性克隆，上清中表达产物经过Tricine-SDS-PAGE鉴定与预期的目的蛋白大小（约10ku）相符．并利用琼脂孔穴扩散法证明了表达产物能抑制革兰氏阳性菌和革兰氏阴性菌的生长．
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| 关 键 词： | 抗菌肽scygonadin 毕赤酵母 分泌表达 |
Recombinant Expression of the Anti-microbial Peptide,Scygonadin from Scylla Paramamosain in Pichia Pastoris and its Antimicrobial Activity |
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| XU Wan-fang,XIE Jia-hua,CHEN Hui-yun.Recombinant Expression of the Anti-microbial Peptide,Scygonadin from Scylla Paramamosain in Pichia Pastoris and its Antimicrobial Activity[J].Journal of Quanzhou Normal College,2011,29(6):11-15. |
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| Authors: | XU Wan-fang XIE Jia-hua CHEN Hui-yun |
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| Institution: | XU Wan-fang1,2,XIE Jia-hua1,CHEN Hui-yun2(1.School of Chemistry and Life Sciences,Quanzhou Normal University,Fujian 362000,China,2.State Key Laboratory of Marine Environmental Science,College of Oceanography and Environmental Science,Xiamen University,Fujian 361005,China) |
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| Abstract: | The mature peptide sequence of antimicropeptide scygonadin in Scylla paramamosain was subcloned into Pichia pastoris expression vector pPIC9K to construct the secretory expression vector pPIC9k-Scy and subsequently transformed into the GS115 yeast strain using the electroporation.The positive clones,of which the chromosomes were integrated with scygonadin gene,were identified using the phenotype screning and PCR.Then they were induced by methanol and the expression product in the supernatant of about 10 ku,... |
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| Keywords: | antibacterial peptide scygonadin Pichia pastoris secretory expression |
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