| 人铁蛋白基因的克隆与原核表达 |
| |
| 引用本文： | 许婉芳,陈曦,陈巧君.人铁蛋白基因的克隆与原核表达[J].泉州师范学院学报,2011,29(4):45-49. |
| |
| 作者姓名： | 许婉芳 陈曦 陈巧君 |
| |
| 作者单位： | 泉州师范学院化学与生命科学学院,福建泉州,362000 |
| |
| 基金项目： | 泉州市科技计划项目(2010Z55) |
| |
| 摘 要： | 通过PCR技术扩增出人铁蛋白基因，经酶切后与表达载体质粒pGEX-4T-2连接，重组质粒转化感受态大肠杆菌，利用菌落PCR、质粒双酶切、测序，证实成功地构建了人铁蛋白基因表达载体，利用IPTG对重组菌进行诱导表达，通过尿素洗涤纯化目的蛋白用于制备抗体．
|
| 关 键 词： | 铁蛋白 重组 表达 |
Cloning and Expression of the Ferritin Gene in Homo Sapiens |
| |
| XU Wan-fang,CHEN Xi,CHEN Qiao-jun.Cloning and Expression of the Ferritin Gene in Homo Sapiens[J].Journal of Quanzhou Normal College,2011,29(4):45-49. |
| |
| Authors: | XU Wan-fang CHEN Xi CHEN Qiao-jun |
| |
| Institution: | XU Wan-fang,CHEN Xi,CHEN Qiao-jun(School of Chemistry and Life Science,Quanzhou Normal University,Fujian 362000,China) |
| |
| Abstract: | The ferritin gene was cloned from human genome using the PCR technology.After digested by the enzymes(BamH I,Sma I),it was inserted into the plasmid pGEX-4T-2 to reconstruct the expression vector.Then the reconstructed plasmid was converted into E.coli whereby to confirm using the colony PCR,the enzyme digestion and the DNA sequencing.The recombinant protein was expressed after inducing by IPTG and then purified using urea for antibody preparation. |
| |
| Keywords: | ferritin reconstruction expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
