| 以β-淀粉样蛋白为靶的表达载体的构建及鉴定 |
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| 引用本文： | 王烈峰,徐敏雯.以β-淀粉样蛋白为靶的表达载体的构建及鉴定[J].赣南师范学院学报,2006,27(3):85-87. |
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| 作者姓名： | 王烈峰 徐敏雯 |
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| 作者单位： | 1. 赣南师范学院,化学与生命科学学院
2. 赣南医学院,附属医院眼科,江西,赣州,341000 |
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| 摘 要： | 饱和苯酚氯仿法提取Tg2576转基因鼠基因组DNA,PCR法扩增编码β-淀粉样蛋白的目的基因,利用基因克隆技术构建以β-淀粉样蛋白为靶的表达载体pcDNA3.1-Aβ42×2,应用酶切及测序鉴定表达载体.相应的双酶酶切能够获得插入的目的基因片段(为269bp),测序未发现突变,表达载体构建成功.
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| 关 键 词： | 老年性痴呆 β-淀粉样蛋白 Tg2576转基因鼠 重组质粒 表达载体 |
| 文章编号： | 1004-8332（2006）03-0085-03 |
| 收稿时间： | 2005-09-09 |
| 修稿时间： | 2005-12-29 |
Construction Eukaryotic Express Vector Aimed Amyloid-β as a Target |
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| WANG Lie-feng,XU Ming-wen.Construction Eukaryotic Express Vector Aimed Amyloid-β as a Target[J].Journal of Gannan Teachers' College(Social Science(2)),2006,27(3):85-87. |
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| Authors: | WANG Lie-feng XU Ming-wen |
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| Abstract: | Saturation Phenol and Chloroform distills the DNA of Tg2576 transgenic mouse.The coding sequence of Aβis amplified by PCR .To construct the express vector aimed Amyloid-β as a target,the gene fragment was cloned directionally into the multi-cloning site of the plasmid and the recombine plasmid was constructed.The eukaryotic express vector is idenutified,according to enzyme digesting and gene sequencing.The goal gene(137bp and 269bp) can be achieved after the restriction analysis.The express vector was constructed successfully. |
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| Keywords: | Alzheimer's disease Amyloid-βprotein Tg2576 transgenic mouse Recombine plasmid express vector |
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