| 鸡源NDV WF00C株F基因的测序与蛋白特性分析 |
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| 引用本文： | 胡元庆,路振香,张训海,金光明,陈会良.鸡源NDV WF00C株F基因的测序与蛋白特性分析[J].安徽科技学院学报,2009,23(1). |
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| 作者姓名： | 胡元庆 路振香 张训海 金光明 陈会良 |
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| 作者单位： | 安徽科技学院动物科学学院,家禽疫病防控监测安徽省重点实验室,安徽凤阳233100;安徽科技学院动物科学学院,家禽疫病防控监测安徽省重点实验室,安徽凤阳233100;安徽科技学院动物科学学院,家禽疫病防控监测安徽省重点实验室,安徽凤阳233100;安徽科技学院动物科学学院,家禽疫病防控监测安徽省重点实验室,安徽凤阳233100;安徽科技学院动物科学学院,家禽疫病防控监测安徽省重点实验室,安徽凤阳233100 |
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| 基金项目： | 国家自然科学基金，安徽省教育厅自然科学基金 |
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| 摘 要： | 用鸡源新城疫病毒凤阳分离株WF00C对9～10 日龄SPF鸡胚的尿囊腔接种,成功增殖了该病毒,采用一步法RT-PCR技术扩增WF00C病毒的F基因,获得了1条1.7kb的特异性条带.用PCR产物直接测序.测序结果表明,扩增片段大小为1782bp,含有1个1662bp的开放性阅读框,编码554个氨基酸.核苷酸同源性分析表明:WF00C株与国内外其他NDV F基因的同源性为84.8%～97.5%,其中与国内标准强毒株F48E9的同源性为87.1%,说明WF00C与国内外的传统毒株有较大变异.与Taiwan95株和J株的同源性为94.1%和97.5%,说明WF00C与Taiwan95株和J株亲缘关系较近,具有较高的相似性.F蛋白裂解位点的氨基酸顺序为112Arg-Arg-Gln-Lys -Arg-Phe117, 表明为NDV的强毒株.蛋白疏水性和抗原性分析表明与标准强毒株相比没有太大的变异.
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| 关 键 词： | 鸡源NDV F基因 序列测定 病毒毒力 同源性分析 |
Sequencing and Protein Analysis of F Gene of NDV WF00C from Chicken |
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| HU Yuan-qing,LU Zhen-xiang,ZHANG Xun-hai,JIN Guang-ming,CHEN Hui-liang.Sequencing and Protein Analysis of F Gene of NDV WF00C from Chicken[J].Journal of Anhui Science and Technology University,2009,23(1). |
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| Authors: | HU Yuan-qing LU Zhen-xiang ZHANG Xun-hai JIN Guang-ming CHEN Hui-liang |
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| Institution: | Animal Science College;Anhui Science and Technology University;Anhui Key Laboratory of Poultry Epidemic Disease Prevention and Surveillance;Fengyang 233100;China |
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| Abstract: | The ND virus of WF00C was grown in 10-day-old SPF embrocated eggs.The F gene of Fengyang strain WF00C of chicken was amplified by one step RT-PCR.The sequencing analysis showed that the sequence of the F gene was 1782bp,encoding a protein of 554 amino acids.The homology between WF00C and the NDV strains inside and outside China was from 84.8%to97.5%.The WF00C F gene's nucleotide sequence shares 87.1% homology with that of the NDV strain F48E9.The ORF of WF00C F gene shares 94.1% and 97.5% nucleotide homolog... |
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| Keywords: | Newcastle Disease Virus from Chicken F Gene Gene Sequencing Virulence of NDV Analysis of Nucleotide Homology |
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