DNA methylation polymorphism in flue-cured tobacco and candidate markers for tobacco mosaic virus resistance |
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| Authors: | Zhao Jie-hong Zhang Ji-shun Wang Yi Wang Ren-gang Wu Chun Fan Long-jiang Ren Xue-liang |
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| Institution: | (1) Department of Horticultural Biotechnology, Kyung Hee University, Seacheon-dong, Giheung-gu, Yongin-Si, Gyeonggi-Do, 446-701, South Korea; |
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| Abstract: | DNA methylation plays an important role in the epigenetic regulation of gene expression during plant growth, development,
and polyploidization. However, there is still no distinct evidence in tobacco regarding the distribution of the methylation
pattern and whether it contributes to qualitative characteristics. We studied the levels and patterns of methylation polymorphism
at CCGG sites in 48 accessions of allotetraploid flue-cured tobacco, Nicotiana tabacum, using a methylation-sensitive amplified polymorphism (MSAP) technique. The results showed that methylation existed at a
high level among tobacco accessions, among which 49.3% sites were methylated and 69.9% allelic sites were polymorphic. A cluster
analysis revealed distinct patterns of geography-specific groups. In addition, three polymorphic sites significantly related
to tobacco mosaic virus (TMV) resistance were explored. This suggests that tobacco breeders should pay more attention to epigenetic
traits. |
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