HPLC method for amino acids profile in biological fluids and inborn metabolic disorders of aminoacidopathies |
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| Authors: | S V Suresh Babu M M Shareef A Pavan Kumar Shetty K Taranath Shetty |
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| Institution: | (1) Clinical Biochemistry Unit, Department of Neurochemistry, National Institute of Mental Health and Neurosciences, 560029 Bangalore |
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| Abstract: | Quantification of total and individual amino acids in biological fluids such as plasma, urine and cerebrospinal fluid has
an important diagnostic implication in laboratory medicine. The present paper describes protocols for the assay of total amino
acids by modified method based on dinitrophenyl and HPLC profile involving pre-column derivatization with o-pthalaldehyde
(OPA) derivatization, respectively. The method, based on the alkylation of-SH groups prior to OPA derivatization of amino
acids followed by reverse phase high performance liquid chromatography, provide a comprehensive profile of more than twenty
amino acids (including-SH group containing) in a single run lasting about 45 minutes. The present study, apart from establishing
the normal profile of amino acids in plasma of Indian sub population, also presents HPLC profile for some of the rare amino
acidopathies. |
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| Keywords: | O-pthaladehyde amino acidopathies amino acids profile reverse phase HPLC Inborn metabolic disorders |
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