An integrated, multiparametric flow cytometry chip using ??microfluidic drifting?? based three-dimensional hydrodynamic focusing |
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| Authors: | Xiaole Mao Ahmad Ahsan Nawaz Sz-Chin Steven Lin Michael Ian Lapsley Yanhui Zhao J Philip McCoy Wafik S El-Deiry Tony Jun Huang |
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| Institution: | 1Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, Pennsylvania 16802, USA;2Department of Bioengineering, The Pennsylvania State University, University Park, Pennsylvania 16802, USA;3National Heart, Lung, and Blood Institute at NIH, Bethesda, Maryland 20892, USA;4Penn State Hershey Cancer Institute, Hershey, Pennsylvania 17033, USA |
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| Abstract: | In this work, we demonstrate an integrated, single-layer, miniature flow cytometry device that is capable of multi-parametric particle analysis. The device integrates both particle focusing and detection components on-chip, including a “microfluidic drifting” based three-dimensional (3D) hydrodynamic focusing component and a series of optical fibers integrated into the microfluidic architecture to facilitate on-chip detection. With this design, multiple optical signals (i.e., forward scatter, side scatter, and fluorescence) from individual particles can be simultaneously detected. Experimental results indicate that the performance of our flow cytometry chip is comparable to its bulky, expensive desktop counterpart. The integration of on-chip 3D particle focusing with on-chip multi-parametric optical detection in a single-layer, mass-producible microfluidic device presents a major step towards low-cost flow cytometry chips for point-of-care clinical diagnostics. |
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