| PCR法分析WSSV在日本对虾体内的感染增殖 |
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| 引用本文： | 吴文林,戴聪杰,王磊.PCR法分析WSSV在日本对虾体内的感染增殖[J].泉州师范学院学报,2005,23(2):77-80. |
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| 作者姓名： | 吴文林 戴聪杰 王磊 |
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| 作者单位： | 1. 泉州师范学院,生物系,福建,泉州,362000
2. 厦门大学,生命科学学院,福建,厦门,361005 |
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| 基金项目： | 国家高新技术研究与发展计划（863计划）项目资助（2003AA603420） |
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| 摘 要： | 注射白斑症病毒（WSSV）感染日本对虾，隔一定时间取样。PCR检测病毒在对虾体内的分布情况．结果表明：感染后12h首先在血液和鳃中出现病毒，而后是消化道和心脏，最后是肌肉和附肢，但在肝胰腺中没有观察到病毒．
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| 关 键 词： | PCR 日本对虾 白斑症病毒 感染增殖 |
| 文章编号： | 1009-8224(2005)02-0077-04 |
| 收稿时间： | 06 25 2004 12:00AM |
| 修稿时间： | 2004年6月25日 |
Infection Development of White Spot Syndrome Virus in Penaeus japonicus |
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| WU Wen-lin,DAI Cong-jie,WANG Lei.Infection Development of White Spot Syndrome Virus in Penaeus japonicus[J].Journal of Quanzhou Normal College,2005,23(2):77-80. |
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| Authors: | WU Wen-lin DAI Cong-jie WANG Lei |
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| Institution: | 1, Department of Biology Sciences, Quanzhou Normal University, Fujian 362000, China; 2, School of Life Sciences, Xiamen University, Xiamen 361005, China |
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| Abstract: | Penaeus japonicus was experimentally infected WSSV by injecting. Different tissues of shrimps were sampled in different hours after infection and detected WSSV by PCR in order to dynamically study the viral infection process in shrimp body. The results indicate that WSSV firstly infect gill or hemolymp, then intestine and heart, lastly muscle and limb,but not infect hepatopancreas. |
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| Keywords: | PCR Penaeus japonicus WSSV infection development |
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