| 氯化血红素与三种芳香族氨基酸的相互作用 |
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| 引用本文： | 刘凡,李琼.氯化血红素与三种芳香族氨基酸的相互作用[J].乐山师范学院学报,2014(5):19-21. |
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| 作者姓名： | 刘凡 李琼 |
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| 作者单位： | 乐山师范学院化学学院,四川乐山614000 |
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| 摘 要： | 利用荧光光谱法研究了氯化血红素(Hemin)与色氨酸(Trp)、酪氨酸(Tyr)和苯丙氨酸(Phe)的相互作用,用Stern-Volmer方程、Lineweaver-Burk方程确定了其荧光猝灭机理为静态猝灭,得到了不同温度和pH条件下的氯化血红素与氨基酸作用的结合常数、结合点位数及作用体系的热力学参数。
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| 关 键 词： | 氯化血红素 色氨酸 酪氨酸 苯丙氨酸 荧光光谱 结合常数 结合位点数 |
The Interaction Between Hemin and Tryptophan,Tyrosine and Phenylalanine |
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| LIU Fan,LI Qiong.The Interaction Between Hemin and Tryptophan,Tyrosine and Phenylalanine[J].Journal of Leshan Teachers College,2014(5):19-21. |
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| Authors: | LIU Fan LI Qiong |
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| Institution: | (School of Chemistry, Leshan Normal University, Leshan Sichuan 614000, China) |
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| Abstract: | The interaction of Hemin with Tryptophan, Tyrosine and Phenylalanine was investigated by fluorescence spectroscopy method. The results show that the Hemin strongly quenches the fluorescence of Tryptophan, Tyrosine and Phenylalanine. It has been proved that the fluorescence quenching mechanism belongs to the static quenching by Stern-Volmer equation and Lineweaver-Burk equation. According to Lineweaver -Burk equation and thermodynamics equation, the binding constants, binding sates and thermodynamics parameters were obtained at different temperatures and pH in the aqueous solution and in the solutions of Gemini surfactants. |
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| Keywords: | Hemin Tryptophan Tyrosine Phenylalanine Fluorescence Spectroscopy Binding Numbers Binding Constants |
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