Estimation of mRNA levels of interleukin (IL)-10, tumor necrosis factor (TNF)-α, IL-4 and interferon (IFN)-γ in HIV infected children in Mumbai |
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| Authors: | Sweta T Kothari Ranjana A Deshmukh |
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| Institution: | (1) Department of Virology, Haffkine Institute for Training, Research and Testing, A. D. Marg, Parel, 12 Mumbai |
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| Abstract: | Cytokines, viral load and opportunistic infections play an important role in HIV-disease progression. Hundred children vertically
infected with HIV were enrolled to determine mRNA levels of TNF-α, IL-10, IL-4 and IFN-γ. These levels were estimated by amplifying
cytokine mRNA from peripheral blood mononuclear cells. Severity of HIV was staged by the reduction in CD4
+ T cells and the onset of opportunistic infections. IL-10 mRNA levels were observed to increase with the severity. Despite
the rising IL-10 mRNA levels, TNF-α mRNA levels increased with severity of HIV and decrease in CD4
+ T cell counts. IL-4 mRNA levels increased with the reduction in CD4
+ T cell numbers. Depleting mRNA levels of IFN-γ contributed to the worsening of HIV disease. Increase in TNF-α and IL-4 levels
appended to the disease severity by upregulation of the viral replication. Increased IL-10 levels and decreased IFN-γ levels
predisposed the children to HIV associated opportunistic infections, which in return contributed to cytokine disarray. |
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| Keywords: | HIV disease severity mRNA levels cytokines children |
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