| LC/MS法测定茶叶中4种儿茶素 |
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| 引用本文： | 王明锋,陈敬玲,王庆忠,廖予琦,张国民.LC/MS法测定茶叶中4种儿茶素[J].昆明师范高等专科学校学报,2010(6):59-61,71. |
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| 作者姓名： | 王明锋 陈敬玲 王庆忠 廖予琦 张国民 |
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| 作者单位： | [1]云南大学化学科学与工程学院,云南昆明650091 [2]红云红河烟草（集团）有限责任公司,云南昆明650202 |
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| 基金项目： | 云南大学骨干教师培训计划资助项目（W3302000） |
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| 摘 要： | 利用Agilent LC/MSD TOF建立了快速测定茶叶中4种主要儿茶素的分析方法.首先以儿茶素对应的质谱峰的强度和质谱峰分辨率为基准,采用正交实验设计方法对质谱联用仪的仪器参数进行了优化选择,包括流速,毛细管高压,干燥气体流速及温度,裂解电压,雾化器压力等;依据优化后的质谱测定条件,应用标准加入法,对实际的样品测定进行了重现性、精密度和回收率的考察;最后对实际的茶叶样品的含量进行了测定,该方法单次进样测量时间为2 min,为快速测定茶叶中儿茶素提供了一种可供参考的分析方法.
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| 关 键 词： | 茶叶 儿茶素 HPLC MS 标准加入法 |
Simultaneous Determination of Four Catechins in Tea by LC/MS |
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| WANG Ming-feng,CHEN Jing-ling,WANG Qing-zhong,LIAO Yu-qi,ZHANG Guo-min.Simultaneous Determination of Four Catechins in Tea by LC/MS[J].Journal of Kunming Teachers College,2010(6):59-61,71. |
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| Authors: | WANG Ming-feng CHEN Jing-ling WANG Qing-zhong LIAO Yu-qi ZHANG Guo-min |
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| Institution: | 1.School of Chemical Science and Technology,Yunnan University,Yunnan Kunming 650091,China; 2.Hongyun and Honghe Tobacco(Group) Limited Company,Yunnan Kunming 650202,China) |
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| Abstract: | A rapid approach of measuring the amount of four catechins in tea was set up by LC/MS（TOF）.Firstly,optimization of instrumental coefficients,such as capillary,drying Gas,fragment,gas temperature,nebulizer and Oct.RF voltage,were done base on both the intensities and resolutions of catechin;then the reproducibility,level of quantization and recovery were investigated;and last,the amount of catechin of actual samples was measured.The procedure can be finished within 2 minutes which provides analysis method for reference on rapid measuring the catechin in tea. |
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| Keywords: | tea catechin HPLC MS the method of standard additions |
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