| Batch and fed-batch production of butyric acid by Clostridium butyricum ZJUCB |
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| 作者姓名： | 何国庆 孔青 陈启和 阮晖 |
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| 作者单位： | Department of Food Science and Nutrition，Zhejiang University，Department of Food Science and Nutrition，Zhejiang University，Department of Food Science and Nutrition，Zhejiang University，Department of Food Science and Nutrition，Zhejiang University Hangzhou 310029，China，Hangzhou 310029，China，Hangzhou 310029，China，Hangzhou 310029，China |
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| 摘 要： | INTRODUCTION Butyric acid has several potential applications in industry. Its applications in the foodstuffs and beverage industries are widespread. It may be used as the pure acid in the dairy industry, or in the form of esters as a food additive to increase fruit fragrance. Furthermore, butyric acid could also have some important physiological functions. For example, butyric acid esters are the character-impact flavors in tropic fruits and dairy products (Centeno et al., 2002; Watson e…
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Batch and fed-batch production of butyric acid by Clostridium butyricum ZJUCB |
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| HE Guo-qing,KONG Qing,CHEN Qi-he,RUAN Hui.Batch and fed-batch production of butyric acid by Clostridium butyricum ZJUCB[J].Journal of Zhejiang University Science,2005,6(11). |
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| Authors: | HE Guo-qing KONG Qing CHEN Qi-he RUAN Hui |
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| Abstract: | The production of butyric acid by Clostridium butyricum ZJUCB at various pH values was investigated. In order to study the effect of pH on cell growth, butyric acid biosynthesis and reducing sugar consumption, different cultivation pH values ranging from 6.0 to 7.5 were evaluated in 5-L bioreactor. In controlled pH batch fermentation, the optimum pH for cell growth and butyric acid production was 6.5 with a cell yield of 3.65 g/L and butyric acid yield of 12.25 g/L. Based on these results, this study then compared batch and fed-batch fermentation of butyric acid production at pH 6.5. Maximum value (16.74 g/L) of butyric acid concentration was obtained in fed-batch fermentation compared to 12.25 g/L in batch fermentation. It was concluded that cultivation under fed-batch fermentation mode could enhance butyric acid production significantly (P＜0.01) by C. butyricum ZJUCB. |
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| Keywords: | Clostridium butyricum Batch fermentation Fed-batch fermentation pH Butyric acid production Acetic acid |
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