| 氧化沟污水处理系统中病原菌的检测与归趋分析 |
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| 作者姓名： | 林雨新 张震南 徐亚慧 李娟 刘新春 |
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| 作者单位： | 中国科学院研究生院资源与环境学院, 北京 100049 |
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| 基金项目： | 国家自然科学基金(50978250)和国家科技支撑计划(2007BAD87B12-3A)资助 |
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| 摘 要： | 利用定量PCR技术,检测氧化沟污水处理系统中各处理单元的病原菌及其指示菌的数量变化状况.检测对象包括大肠杆菌、沙门氏菌和军团菌.结果表明,大肠杆菌uidA基因的进水浓度在108拷贝数/mL左右,从进水到出水减少了2个数量级;沙门氏菌invA基因的进水浓度在102～103拷贝数/mL左右,数量较少,并在后续污水处理过程中减少到检出限以下;军团菌16SrDNA基因在氧化沟内检出量较小,大约是10～102,但在出水样品中的数量与进水差别不大,在104～105拷贝数/mL左右.
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| 关 键 词： | 氧化沟 病原菌 实时荧光定量PCR |
| 收稿时间： | 2012-01-13 |
| 修稿时间： | 2012-03-27 |
Detection of selected pathogenic bacteria during oxidation ditch wastewater treatment |
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| Authors: | LIN Yu-Xin ZHANG Zhen-Nan XU Ya-Hui LI Juan LIU Xin-Chun |
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| Institution: | College of Resources and Environment, Graduate University, Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Indicator bacterium E. coli and pathogenic bacteria Salmonella and Legionella were detected during the wastewater treatment of oxidation ditch, using SYBR I Real-time quantitative PCR. The detected amount of E.coli was 108 copies/mL and decreased by 2 orders of magnitude eventually. In the initial stages, the amount of Salmonella ranged from 102 to 103 copies/mL but was eliminated or reduced to undetectable level eventually. The amount of Legionella was detected to be 10-102 copies/mL in the sample of activated sludge. However, the amount of Legionella in the effluent samples was detectable and similar to those in the influent samples, which ranged from 104 to 105 copies/mL. |
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| Keywords: | oxidation ditch pathogenic bacteria real-time quantitative PCR |
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