| Improved production of spiramycin by mutant Streptomyces ambofaciens |
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| 作者姓名： | 金志华 岑沛霖 |
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| 作者单位： | Department of Biological and Pharmaceutical Engineering，Ningbo Institute of Technology，Zhejiang University，Institute of Bioengineering，College of Materials Science and Chemical Engineering，Zhejiang University Ningbo 315100，China，Institute of Bioengineering，College of Materials Science and Chemical Engineering，Zhejiang University，Hangzhou 310027，China，Hangzhou 310027，China |
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| 摘 要： | INTRODUCTION which the hydroxyl group is acetylated, and spi- ramycin III in which the same position is propio- Spiramycin belongs to the macrolide antibi- nylated (Omura et al., 1979). The addition of short-otics group. The molecular structure of spiramycin chain fatty acids stimulates the production of spi-consists of a 16-membered branched lactonic ring …
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Improved production of spiramycin by mutant Streptomyces ambofaciens |
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| JIN Zhi-hua ,CEN Pei-lin.Improved production of spiramycin by mutant Streptomyces ambofaciens[J].Journal of Zhejiang University Science,2004(6). |
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| Authors: | JIN Zhi-hua CEN Pei-lin |
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| Institution: | JIN Zhi-hua 1,2,CEN Pei-lin 2 |
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| Abstract: | Strain improvement and medium optimization to increase the productivity of spiramycin were carried out. Of oil tolerant mutant strains screened, one mutant, Streptomyces ambofaciens XC 2-37, produced 9% more spiramycin than the parent strain S. ambofaciens XC 1-29. The effects of soybean oil and propyl alcohol on spiramycin production with S. ambofaciens XC 2-37 were studied. The potency of S. ambofaciens XC 2-37 was improved by 61.8% with addition of 2% soybean oil in the fermentation medium and 0.4% propyl alcohol at 24 hours after incubation. The suitable time for feeding propyl alcohol is at 24 hours after incubation in flask fermentation and at 20 hours after incubation in fermentor fermentation. The new process with S. ambofaciens XC 2-37 was scaled up for industrial scale production of spiramycin in a 60 m3 fermentor in Xinchang Pharmaceutical Factory, Zhejiang Medicine Company, Ltd., China, and the potency and productivity of fermentation were improved by 42.9%. |
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| Keywords: | Spiramycin Streptomyces ambofaciens Strain improvement Fermentation |
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