Cloning and characterization of a glucose 6-phosphate/phosphate translocator from<Emphasis Type="Italic">Oryza sativa</Emphasis> |
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| Authors: | Jiang Hua-wu Dian Wei-min Liu Fei-yan Wu Ping |
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| Institution: | (1) The State Key Laboratory of Plant Physiology and Biochemistry, College of Life sciences, Zhejiang University, 310029 Hangzhou, China |
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| Abstract: | Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6-phosphate is the
preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6-phosphate/phosphate translocator
(GPT) was isolated from a cDNA library of immature seeds of rice and named asOsGPT. The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of
70 amino acid residues. TheOsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000-grain weight. The expression ofOsGPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6-phosphate imported viaGPT can be used for starch biosynthesis in rice nongreen plastids.
Project supported by National Natural Scienc Foundation of China (No.39830250) and Natural Science Foundation of Zhejiang
Province (No.2A0106), China. The nucleotide sequence data will appear in the GenBank under accession number AF375053. |
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| Keywords: | Glucose 6-phosphate/phosphate translocator Starch synthesis Rice (Oryza sativa L ) |
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