| 金银花中绿原酸和木犀草苷的同时提取及测定研究 |
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| 引用本文： | 王海侠,汪玮鑫,时维静,郜尽.金银花中绿原酸和木犀草苷的同时提取及测定研究[J].安徽科技学院学报,2011,25(5):37-41. |
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| 作者姓名： | 王海侠 汪玮鑫 时维静 郜尽 |
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| 作者单位： | 安徽科技学院理学院,安徽凤阳,233100;安徽科技学院生命科学学院,安徽凤阳,233100;上海交通大学药学院,上海,200240 |
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| 基金项目： | 安徽省教育厅优秀人才基金项目(2006JQ1196); 安徽科技学院引进人才专项(ZRC200684),安徽科技学院重点建设学科(AKXK20102-2) |
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| 摘 要： | 目的：优化金银花样品中绿原酸和木犀草苷提取工艺，并建立两者含量同时测定的方法。方法：设计三水平四因素的正交试验，筛选最佳提取条件，并以此条件处理不同产地的金银花，用HPLC测定其绿原酸和木犀草苷的含量。结果：最佳提取工艺条件为：用50％的乙醇35mL在70℃下回流提取1h；0．5％冰醋酸和甲醇为流动相，梯度洗脱，检测波长350nm，绿原酸和木犀草苷的线性方程Y=1×106x-8067和Y=4×106X-119017，线性区间为：0．2-1．6ug和O．22-2．21ug，回归系数r值为0。9999和0．9997；精密度、稳定性、回收率试验结果均良好；五个产地的金银花有四个绿原酸和木犀草苷的含量高于药典规定。
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| 关 键 词： | 金银花 HPLC 绿原酸 木犀草苷 产地 |
Study on Simultaneous Extraction Technology and Determination of Chlorogenic Acid and Galuteolin in Flos lonicerae |
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| WANG Hai-xia,WANG Wei-xin,SHI Wei-jing,GAO Jin.Study on Simultaneous Extraction Technology and Determination of Chlorogenic Acid and Galuteolin in Flos lonicerae[J].Journal of Anhui Science and Technology University,2011,25(5):37-41. |
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| Authors: | WANG Hai-xia WANG Wei-xin SHI Wei-jing GAO Jin |
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| Institution: | WANG Hai-xia1,WANG Wei-xin1,SHI Wei-jing2,GAO Jin3(1.College of Science,Anhui Science and Technology University,Fengyang 233100,China,2.College of Life Science,3.School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China) |
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| Abstract: | Objective: To optimalize the extracting conditions of chlorogenic acid and galuteolin from Flos lonicerae,and develop one method for the content determination of chlorogenic acid and galuteolin.Methods: Through designing a L9(34)orthogonal test to screen the best extracting parameters and extract chlorogenic acid and galuteolin in Flos lonicerae of the five different production regions under the condition.Then a HPLC method was dopted to determine the content of those samples.Results: Orthogonal experiment ... |
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| Keywords: | Lonicera japonica HPLC Chlorogenic acid Galuteolin Production region |
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