| 盘龙参组织培养的初步研究 |
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| 引用本文： | 李家敏.盘龙参组织培养的初步研究[J].宜春学院学报,2011,33(8). |
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| 作者姓名： | 李家敏 |
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| 作者单位： | 宜春学院化学与生物工程学院,江西宜春,336000 |
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| 基金项目： | 江西省教育厅青年基金项目GJJ10252，江西省教育厅项目GJJ08410，宜春市社会发展科技计划JXYC2010KSB004 |
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| 摘 要： | 以盘龙参（Spiranthes sinensis）的花梗为外植体,探讨了不同前灭菌处理及植物生长调节剂对盘龙参花梗组织培养的影响。结果表明：75%的酒精浸30s的前灭菌处理效果较好;植物生长调节剂组合6-BA0.1（mg/L）＋NAA1（mg/L）组合和6-BA1.5（mg/L）＋NAA1（mg/L）组合的效果较好,其生存率分别达到了60%和66%。
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| 关 键 词： | 盘龙参 花梗 灭菌 植物生长调节剂 初代培养 |
The Preliminary Study of Tissue Culture in Spiranthes sinensis |
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| LI Jia-min.The Preliminary Study of Tissue Culture in Spiranthes sinensis[J].Journal of Yichun University,2011,33(8). |
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| Authors: | LI Jia-min |
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| Institution: | LI Jia-min(College of Chemical and Biological Engineering,Yichun University,Yichun 336000 China) |
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| Abstract: | the effects of different pre-bactericidal treatment and the combinations of plant growth regulators on the Spiranthes sinensis inflorescence tissue culture were studied.The results indicated that the best method for pre-bactericidal treatment was soaking the material in 70% alcohol for 30 seconds.The optimum plant growth regulator combinations were 6-BA 0.1（mg/L）＋NAA 1（mg/L） and 6-BA 1.5（mg/L）＋NAA 1（mg/L） on which living rates were 60% and 66% respectively. |
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| Keywords: | Spiranthes sinensis inflorescence bactericidal treatment plant growth regulator primary culture |
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