| 基于褐藻酸钠改性纳米金静电排斥作用的新型电化学免疫传感器的研制 |
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| 引用本文： | 胡舜钦,谢志美,雷存喜.基于褐藻酸钠改性纳米金静电排斥作用的新型电化学免疫传感器的研制[J].湖南城市学院学报,2003,24(3):97-99. |
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| 作者姓名： | 胡舜钦 谢志美 雷存喜 |
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| 作者单位： | 1. 株洲师范高等专科学校,化学系,湖南,株洲,412007
2. 湖南城市学院,化学与环境工程系,湖南,益阳,413049 |
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| 摘 要： | 通过胱胺将负电性的褐藻酸钠(AS)共价结合到纳米金表面形成聚合体，并以此来标记三碘甲状腺氨酸(T3)．T3抗体固定在金电极上构成检测T3的电化学免疫传感器．通过免疫反应，标记T3和待检测的T3结合到金电极表面，使电极表面微环境发生显著改变，用Fe(CN)6^4 为电化学探针，用循环伏安法获取电极表面微环境改变的电化学信息来检测T3、其检测的线性范围为100—10000pg／mL，检测限为45pg/mL.
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| 关 键 词： | 电化学免疫传感器 褐藻酸钠 纳米金 静电排斥作用 三碘甲状腺氨酸 电极表面微环境 |
| 文章编号： | 1672-1942(2003)03-0097-03 |
| 修稿时间： | 2002年11月21 |
A New Desiging Approach to Electrochemical Immuno-sensor Based on Electrostatic Reject of Gold Nanoparticles Modified with Alginates |
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| HU Shun-qin,XIE Zhi-mei,LEI Cun-xi.A New Desiging Approach to Electrochemical Immuno-sensor Based on Electrostatic Reject of Gold Nanoparticles Modified with Alginates[J].Journal of Hunan City Univeristy,2003,24(3):97-99. |
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| Authors: | HU Shun-qin XIE Zhi-mei LEI Cun-xi |
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| Institution: | HU Shun-qin1,XIE Zhi-mei1,LEI Cun-xi2 |
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| Abstract: | The aggregate, which was composed of gold nanoparticles modified with alginate via cystamine, was used as a label for T3. The electrochemical immuno-sensor was obtained by covalently immobilized antibody against T3 on the surface of gold electrode. Both labeled and analyte T3 were anchored on the immuno-sensor because of reaction between T3 and its antibody. This led to the changes of micro- environment of the surface of immuno-sensor. The changes, that were indicated by cyclic voltammetry technology using Fe (CN)64- as the electrochemical probe, was corresponding to the reaction between T3 and its antibody. The proposed immuno-sensor was successfully applied to monitor the concentration of T3. The dynamic range of determination was 100~10 000 pg/mL with a detection limit of defermination was 45 pg/mL. |
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| Keywords: | electrochemical immuno-sensor alginate gold nanoparticles micro-environment T3 |
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