| 运动和饮食调整对非酒精性脂肪肝大鼠内质网应激PERK／eIF－2a和IRE－1／XBP－1通路的影响 |
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| 引用本文： | 李军汉,孙君志,李恩,张仲阳,苏全生.运动和饮食调整对非酒精性脂肪肝大鼠内质网应激PERK／eIF－2a和IRE－1／XBP－1通路的影响[J].成都体育学院学报,2016(6). |
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| 作者姓名： | 李军汉 孙君志 李恩 张仲阳 苏全生 |
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| 作者单位： | 1. 成都体育学院运动医学与健康学院，四川 成都 610041; 北京体育大学研究生院，北京 100081;2. 成都体育学院运动医学与健康学院,四川 成都,610041;3. 雅安职业技术学院,四川 雅安,625018 |
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| 基金项目： | “十二五”国家科技支撑计划重点项目(2012BAK21B01)；四川省科技厅应用基础计划项目(2015JY0155)；国家体育总局运动医学重点实验室暨运动医学四川省重点实验室资助基金( CTYY2015018)。 |
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| 摘 要： | 目的：观察内质网应激 PERK／ eIF －2a 和 IRE －1／ XBP －1通路在非酒精性脂肪肝（NAFLD）中的变化，探讨运动和饮食调整对非酒精性脂肪肝的干预作用及作用机理。方法：SD雄性大鼠随机分为对照组（20只）和模型组（50只）2组，对照组给予普通饲料喂养，模型组给予高脂饲料喂养。第8周，对照组和模型组各取10只做肝脏病理切片。确定 NAFLD模型成功后，模型组随机分为4组：模型组（M）、运动组（EM）、饮食调整组（FM）和运动结合饮食调整组（ EFM），每组各10只，对照组剩余10只大鼠编为 C组。C组继续普通饲料喂养，M 组和 EM 组继续高脂饲料喂养，FM组和 EFM组由高脂饲料改为普通饲料喂养；EM 组、EFM 组给予有氧游泳运动干预，连续8周，直至第16周实验结束。HE 染色观察肝脏病理形态，Western blot 检测PERK、eIF －2a和 IRE －1、XBP －1蛋白表达，结果：（1）与 C组比较，M组肝细胞脂肪变性加重， PERK／ eIF －2a和 IRE －1／ XBP －1通路蛋白表达增加；（2）与 M组比较，各干预组肝细胞脂肪变性减轻，PERK／ eIF －2a和 IRE －1／ XBP －1通路蛋白表达降低；（3）M 组、EM 组、FM 组、EFM 组4组双因素方差结果示：运动和饮食对 PERK／ eIF －2a 和 IRE －1／ XBP －1通路蛋白表达具有主效应，且二者具有交互作用。结论：运动和饮食调整对 NAFLD 发展具有较好的干预作用，且二者联合作用效果更佳，其机制可能与其降低 PERK／ eIF －2a 和 IRE －1／ XBP －1通路蛋白表达，减少内质网应激有关。
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| 关 键 词： | 内质网应激 非酒精性脂肪肝 运动 饮食 |
The Effect of Exercise and Dietary Modification on PERK/eIF - 2a and IRE - 1/XBP - 1 Pathway Induced by Endoplasmic Reticulum Stress in Nonalcoholic Fatty Liver Disease |
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| LI Junhan,SUN Junzhi,LI En,ZHANG Zhongyang,SU Quansheng.The Effect of Exercise and Dietary Modification on PERK/eIF - 2a and IRE - 1/XBP - 1 Pathway Induced by Endoplasmic Reticulum Stress in Nonalcoholic Fatty Liver Disease[J].Journal of Chengdu Physical Education Institute,2016(6). |
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| Authors: | LI Junhan SUN Junzhi LI En ZHANG Zhongyang SU Quansheng |
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| Abstract: | Objective:To observe the change of eIF - 2a PERK/ and IRE - 1 / XBP - 1 pathway induced by endoplas-mic reticulum stress in nonalcoholic fatty liver disease and explore the role of exercise and dietary modification and its mechanism. Methods:12 rats were randomly selected from 42 rats as the control group,the rest 30 rats were the model group. Control group was fed with ordinary diet,model group was fed with high - fat diet. At the 8th week of experiment,6 rats in control and model group were respectively chose for liver pathological to determine whether NAFLD model was successfully made. Model group were randomly divided into 4 groups:model group (M),exercise group (EM),diet modification group (FM)and exercise combined with diet modi-fication group (EFM),each 6 rats. The remaining 6 rats of control group were as C group . Group C was con-tinued to ordinary diet. Group M and EM were continued to high fat diet. Group FM and EFM were fed from high fat diet to ordinary diet. Group EM and EFM were intervened by aerobic swimming exercise for 8 weeks until the end of experiment. We observed the pathological changes in liver tissue by HE staining. Western blot was employed to explore the protein expression of eukaryotic initiation factor 2 α(eIF - 2a)and protein kinase R - like endoplasmic reticulum kinase (PERK)and inositol - requiring Enzyme 1(IRE - 1)and X - box -binding Protein 1(XBP - 1 ). Results:(1)Compared to group C,hepatocyte steatosis is aggravating and pro-tein expression of PERK/ eIF - 2a and IRE - 1 / XBP - 1 pathway gene increased in M group;(2)Compared to group M,liver steatosis relived and protein expression of PERK/ eIF - 2a and IRE - 1 / XBP - 1 pathway gene reduced in intervention groups;(3)The result of two - way anova in Group M and EM and FM and EFM showed that exercise and diet had a main effect on protein expression of PERK/ eIF - 2a and IRE - 1 / XBP - 1 pathway gene and they had a interactive effect. Conclusions:Exercise and diet modification had an good effect on NAFLD and the effect of exercise combined with diet modification was better,which may be related to the decrease of protein expression of PERK/ eIF - 2a and IRE - 1 / XBP - 1 pathway gene and reducing endoplasmic reticulum stress. |
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| Keywords: | Endoplasmic Reticulum Stress Non - alcoholic Fatty Liver Disease Exercise Diet Modification |
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