Development and evaluation of immunoassay for zeranol in bovine urine |
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| Authors: | Liu Yuan Zhang Cun-zhen Yu Xiang-yang Zhang Zhi-yong Zhang Xiao Liu Rong-rong Liu Xian-jin Gong Zhen-ming |
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| Institution: | (1) Institute of Food Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China;(2) School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200030, China |
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| Abstract: | A high affinity polyclonal antibody-based enzyme linked immunosorbent assay (ELISA) was developed for the quantification of
zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution factors producing insignificant matrix
interference were selected as 1:5 in pretreatment. In the improved ELISA, the linear response range was between 0.02 and 1
μg/ml, and the detection limit was 0.02 μg/ml for the assay. The overall recoveries and the coefficients of variation (CVs)
were in the range of 82%∼127% and 3.5%∼8.8%, respectively. Thirty-six bovine urine samples spiked with zeranol (ranging from
0.2 to 10 μg/ml) were detected by the ELISA and liquid chromatography (LC) method, and good correlations were obtained between
the two methods (R
2=0.9643). We conclude that this improved ELISA is suitable tool for a mass zeranol screening and can be an alternative for
the conventional LC method for zeranol in bovine urine.
Project supported by the National Natural Science Foundation of China (No. 30471155) and the Agriculture Key Technologies
R & D Program of Shanghai (No. (2003) 9-4), China |
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| Keywords: | Zeranol Enzyme linked immunosorbent assay (ELISA) Bovine urine |
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