| 酸-酶结合法提取牛肌腱胶原纤维的研究 |
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| 引用本文： | 贾淑平,吴勇川.酸-酶结合法提取牛肌腱胶原纤维的研究[J].喀什师范学院学报,2012,33(3):56-59. |
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| 作者姓名： | 贾淑平 吴勇川 |
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| 作者单位： | 喀什师范学院化环系,新疆喀什,844008 |
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| 摘 要： | 以牛肌腱为原料,分别利用酸-酶结合法和酶法从冻干碎块中提取胶原纤维,经过盐析、透析、离心和冷冻干燥后制备得到高纯度胶原纤维.通过测定胶原纤维中羟脯氨酸的含量,得到酸-酶结合法的得率为44.9%,远大于单独用酶提取胶原纤维的得率33.8%.对制备的胶原纤维进行红外扫描和差示量热扫描(DSC),结果表明胶原纤维保持了完整的三股螺旋结构,DSC扫描结果表明冻干胶原纤维的变性温度为60.8℃.
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| 关 键 词： | 牛肌腱 酸-酶结合法 胶原纤维 得率 |
Study on Extracting Collagen Fibers of Bovine Tendonwith Acid - Enzyme Binding Assay |
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| JIA Shu-ping,WU Yong-chuan.Study on Extracting Collagen Fibers of Bovine Tendonwith Acid - Enzyme Binding Assay[J].Journal of Kashgar Teachers College,2012,33(3):56-59. |
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| Authors: | JIA Shu-ping WU Yong-chuan |
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| Institution: | (Kashgar Teachers College,Kashgar 844008,Xinjiang) |
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| Abstract: | The high purity collagen fibers was obtained after salting out,dialysis,centrifugation and freeze-dried.Through determining the hydroxyproline content of the collagen fibers,it was found that the collagen fibers of the Acid - enzyme binding with 44.9% much higher than that of enzymatic alone with33.8%.The results of the collagen fibers infrared scanning and Differential Scanning Calorimetry(DSC) showed that the collagen fibers was provided with the complete triple helix structure.And the DSC scan results showed that the denaturation temperature of the freeze-dried collagen fibers was 60 ℃. |
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| Keywords: | Bovine tendon Acid - enzyme collagen fibers Yield |
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