| VEGFmRNA and eNOSmRNA expression in immature rabbits with bleomycin-induced pulmonary hypertension |
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| 作者姓名： | 龚方戚 林幼敏 汤宏峰 顾伟忠 汪伟 康曼丽 |
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| 作者单位： | Affiliated Children’s Hospital，College of Medicine，Zhejiang University，Hangzhou 310003，China，Affiliated Children’s Hospital，College of Medicine，Zhejiang University，Hangzhou 310003，China，Affiliated Children’s Hospital，College of Medicine，Zhejiang University，Hangzhou 310003，China，Affiliated Children’s Hospital，College of Medicine，Zhejiang University，Hangzhou 310003，China，Affiliated Children’s Hospital，College of Medicine，Zhejiang University，Hangzhou 310003，China，Affiliated Children’s Hospital，College of Medicine，Zhejiang University，Hangzhou 310003，China |
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| 基金项目： | Project (No. 300528) supported by the Natural Science Founda-
tion of Zhejiang Province，China |
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| 摘 要： | INTRODUCTION Pulmonary hypertension is not rare in humanwith some heart and lung diseases. Chronic pul-monary hypertension leads to structural alterationsof the lung vessels. The pathophysiology of thisremodeling process is still poorly understood.Furthermore, the structural damage of the lungvessels limits the clinical success of vasodilatortreatment. Assuming genetic susceptibility, shearstress and inflammation are the principal pathoge-netic factors involved in lung vessel remode…
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VEGFmRNA and eNOSmRNA expression in immature rabbits with bleomycin-induced pulmonary hypertension |
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| GONG Fang-qi LIN You-min TANG Hong-feng,GU Wei-zhong WANG Wei KANG Man-li.VEGFmRNA and eNOSmRNA expression in immature rabbits with bleomycin-induced pulmonary hypertension[J].Journal of Zhejiang University Science,2004(8). |
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| Authors: | GONG Fang-qi LIN You-min TANG Hong-feng GU Wei-zhong WANG Wei KANG Man-li |
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| Abstract: | Objective: To investigate the evolution of pulmonary hypertension, the pathological changes of pulmonary arteries, and the expression of VEGFmRNA and eNOSmRNA of pulmonary arterial endothelial cells in immature rabbits treated with intratracheal bleomycin (BLM). Methods: Immature rabbits were divided into control and BLM group. Two and four weeks after intratracheal normal saline or BLM injection, the systolic, diastolic and mean pulmonary artery pressure (PASP, PADP, MPAP) were measured by micro-catheter; the pathological changes and the expression of VEGFmRNA and eNOSmRNA of endothelial cells in pulmonary arteries were evaluated by HE and in situ hybridization. Results: Two and four weeks after intratracheal injection of BLM, the PASP, PADP and MPAP increased 53%, 49%, 52% in 2 weeks, and 43%, 89%, 56% in 4 weeks; the wall thickness increased and the cavity in middle and small pulmonary arteries became narrow; the Thickness Index (TI) and Area Index (AI) increased 25%, 14% in 2 weeks, and 22%, 24% in 4 weeks; the level of VEGFmRNA and eNOSmRNA expression decreased 46%, 43% in 2 weeks, and 43%, 51% in 4 weeks. There was no significant difference between 2 weeks and 4 weeks BLM groups. Conclusion: The pulmonary artery pressure was elevated, the thickness of wall increased and the cavity became narrow in middle and small pulmonary arteries, and the level of VEGFmRNA and eNOSmRNA expression in pulmonary arterial endothelial cells decreased in immature rabbits after 2 weeks and 4 weeks of intratracheal 4 U/kg BLM injection. |
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| Keywords: | Bleomycin Pulmonary hypertension VEGF eNOS mRNA |
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