| 基于DNA熔解温度设计新型基因探针的探索 |
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| 引用本文： | 陈泽琴,豆兴茹,谢洪平.基于DNA熔解温度设计新型基因探针的探索[J].常熟理工学院学报,2011,25(4):56-60. |
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| 作者姓名： | 陈泽琴 豆兴茹 谢洪平 |
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| 作者单位： | 1. 苏州大学医学部药学院,江苏苏州,215123;西华师范大学化学化工学院,四川南充,637002
2. 苏州大学医学部药学院,江苏苏州,215123 |
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| 基金项目： | 国家自然科学基金(81001686)资助项目 |
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| 摘 要： | 应用计算熔解温度的方法,探索应用该方法设计新型基因探针的可能性.利用荧光检测技术进行靶DNA与错配DNA链的鉴别,从而研究了DNA熔解温度对设计新型基因探针的影响.
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| 关 键 词： | 熔解温度 基因探针 荧光检测 |
A Research on Design of Novel Probe Based on Melting Temperature of DNA |
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| CHEN Ze-qin,DOU Xing-ru,XIE Hong-ping.A Research on Design of Novel Probe Based on Melting Temperature of DNA[J].Journal of Changshu Institute of Technology,2011,25(4):56-60. |
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| Authors: | CHEN Ze-qin DOU Xing-ru XIE Hong-ping |
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| Institution: | CHEN Ze-qin1,2,DOU Xing-ru1,XIE Hong-ping1(1.College of Pharmaceutical Sciences,Soochow University,Suzhou 215123,China,2.College of Chemistry and Chemical Engineering,China West Normal University,Nanchong 637002,China) |
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| Abstract: | A research on the possibility to design novel probes by using the method of calculating melting temperature has been done.The authors of this paper use fluorescence detection to identity the target and one-base mismatched DNA.By doing this,the authors want to study the influence of melting temperature on the design of novel probes. |
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| Keywords: | melting temperature(Tm) probe fluorescence detection |
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