| 运用高通量测序研究Alport综合征多能干细胞的microRNA及其靶基因(英文) |
| |
| 作者姓名： | Wen-biao CHEN Jian-rong HUANG Xiang-qi YU Xiao-cong LIN Yong DAI |
| |
| 作者单位： | Clinical Medical Research Center,Shenzhen People’s Hospital;the Second Clinical Medical College of Jinan University;Department of Hemodialysis,the Third People’s Hospital of Shenzhen;Institute of Biochemistry and Molecular Biology,Guangdong Medical College |
| |
| 基金项目： | supported by the Shenzhen Knowledge Innovation Program of Basic Research Items of Guangdong Province(No.JCYJ2014 0416122812045),China |
| |
| 摘 要： | 目的:寻找来源于Alport综合征患者与正常对照者的多能干细胞差异性表达的microR NA,并对差异性表达的microR NA靶基因进行预测。创新点:本研究不同于一般的试验标本,试验标本是来源于尿肾脏管细胞诱导而成的多能干细胞。基于Alport综合征是遗传疾病,我们对一遗传家系进行了系统的分析。寻找特异性的差异性表达microR NA及其靶基因,从基因水平对Alport综合征进行研究。方法:在前期工作中,成功地从实验者与对照者的尿肾脏管细胞诱导成多能干细胞。运用高通量测序技术分析并发现实验者与对照者之间差异性表达的microR NA。对差异性表达的microR NA靶基因进行预测,并进行靶基因聚集分析,研究靶基因主要参与的生物学过程。同时进行靶基因信号传导通路的分析,研究靶基因主要参与的细胞信号传导通路。结论:在实验组与对照组之间,发现了30个具有显著差异性表达的microR NAs,包括19个上调表达与11个下调表达。差异性表达的microR NA的靶基因主要聚集在细胞膜和细胞代谢过程;靶基因主要参与嘌呤代谢通路与丝裂原活化蛋白激酶(MAPK)通路。
|
| 关 键 词： | Alport综合征 多能诱导干细胞 高通量测序 |
| 收稿时间： | 2014 Oct 14 |
Identification of microRNAs and their target genes in Alport syndrome using deep sequencing of iPSCs samples
|
| |
| Wen-biao CHEN,Jian-rong HUANG,Xiang-qi YU,Xiao-cong LIN,Yong DAI.Identification of microRNAs and their target genes in Alport syndrome using deep sequencing of iPSCs samples
[J].Journal of Zhejiang University Science,2015,16(3):235-250,166. |
| |
| Authors: | Wen-biao CHEN;Jian-rong HUANG;Xiang-qi YU;Xiao-cong LIN;Yong DAI;Clinical Medical Research Center Shenzhen People’s Hospital the Second Clinical Medical College of Jinan University; |
| |
| Institution: | Department of Hemodialysis,the Third People’s Hospital of Shenzhen;Institute of Biochemistry and Molecular Biology,Guangdong Medical College; |
| |
| Abstract: | |
| |
| Keywords: | Alport syndrome miRNA Gene Ontology Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway Induced pluripotent stem cells (iPSCs) Solexa sequencing |
| 本文献已被 CNKI 等数据库收录！ |
