| 毛细管电泳安培检测法测定山楂中的有效成分 |
| |
| 引用本文： | 彭友元,张英武.毛细管电泳安培检测法测定山楂中的有效成分[J].泉州师范学院学报,2011,29(2):65-68. |
| |
| 作者姓名： | 彭友元 张英武 |
| |
| 作者单位： | 泉州师范学院,化学与生命科学学院,福建,泉州,362000 |
| |
| 基金项目： | 福建省教育厅A类科研项目，教育部归国留学人员科研启动基金 |
| |
| 摘 要： | 用毛细管电泳安培检测法同时测定山楂中的芦丁、金丝桃甙和绿原酸的含量.考察了实验条件包括电极电位、运行缓冲液的酸度和浓度、电泳电压及进样时间对三组分分离和检测的影响.在实验条件为：以碳圆盘电极（直径300μm）为检测电极,检测电位为＋0.95 V（vs.SCE）,在50 mmoL/L硼酸盐（pH为9.2）的运行缓冲液中,芦丁、金丝桃甙和绿原酸在20 min内能完全分离.被测物浓度与响应电流在三个数量级范围内呈较好的线性,检测限分别为1×10-8、1×10-8和2×10-8g/mL.
|
| 关 键 词： | 山楂 毛细管电泳 安培检测 |
Determination of Active Ingredients from Hawthorn by Capillary Electropheresis with Amperometric Detection |
| |
| PENG You-yuan,ZHANG Yin-wu.Determination of Active Ingredients from Hawthorn by Capillary Electropheresis with Amperometric Detection[J].Journal of Quanzhou Normal College,2011,29(2):65-68. |
| |
| Authors: | PENG You-yuan ZHANG Yin-wu |
| |
| Institution: | PENG You-yuan,ZHANG Yin-wu(School of Chemistry and Life Sciences,Quanzhou Normal University,Fujian 362000,China) |
| |
| Abstract: | a simultaneous determination of active ingredients from hawthorn by capillary electrophoresis with amperometric detection（CE-AD）was reported.The conditions about the experiments were investigated.Under the optimum conditions,the analytes could be separated in a 50 mmoL/L borate buffer（pH=9.2）within 20 min.The response was linear over three orders of magnitude,and the detection limits（S/N=3） were 1×10-8g/mL,1×10-8 g/mL and 2×10-8 g/mL seperately for the analytes.This method has been used for the determination of the analytes from hawthorn,and the assay result is satisfactory. |
| |
| Keywords: | hawthorn capillary electrophoresis amperometric detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
