| 铁皮石斛组培苗复壮培养基的研究 |
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| 引用本文： | 黄作喜,陈东波,贾黄,肖小君,周天慧.铁皮石斛组培苗复壮培养基的研究[J].内江师范学院学报,2014(6):52-54. |
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| 作者姓名： | 黄作喜 陈东波 贾黄 肖小君 周天慧 |
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| 作者单位： | [1]内江师范学院“特色农业资源研究与利用”省高校重点实验室,四川内江641199 [2]内江市公共交易服务中心,四川内江641000 [3]四川先拓生物科技有限公司,四川内江641110 |
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| 基金项目： | 四川省教育厅重点项目（13ZA0007） |
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| 摘 要： | 铁皮石斛组培苗经过多次继代之后,退化严重.利用不同的外源激素及配比、不同浓度Co2＋离子和不同的天然添加物,优化组培苗的培养基.结果表明,MS＋2.0mg/L6-BA（6-苄基腺嘌呤）＋0.10mg/L NAA（萘乙酸）为较好的复壮激素配方,采用1.250倍正常Co2＋浓度、150g/L香蕉汁的培养基,对组培苗的复壮效果较明显.
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| 关 键 词： | 铁皮石斛 组培苗 优化 |
On the Rejuvenation Medium of Tissue Culture Seedlings of Dendrobium officinate Kimura et Migo |
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| HUANG Zuo-xi,CHEN Dong-bo,JIA Huang,XlAO Xiao-jun,ZHOU Tian-hui.On the Rejuvenation Medium of Tissue Culture Seedlings of Dendrobium officinate Kimura et Migo[J].Journal of Neijiang Teachers College,2014(6):52-54. |
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| Authors: | HUANG Zuo-xi CHEN Dong-bo JIA Huang XlAO Xiao-jun ZHOU Tian-hui |
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| Institution: | 1. Key Laboratory of Colleges and Universities for Research and Utilization of Distinctive Agricultural Undertakings, Neijiang Normal University, Neijiang 641199, China 2. Service Center of Public Trading in Neijiang, Neijiang 641000, China 3. Sichuan Xiantuo Bio-technology Co. , Ltd. Neijiang 641000, China) |
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| Abstract: | The tissue culture seedlings ofDendrobium officinate Kimura et Migo became seriously degraded after repeated successive transfer cultures. The medium could be optimized when different kinds of hormones, natural agents as well as Co2＋ in different concentrations and mixture ratios were used to cultivate seedlings. The results showed that MS ＋2.0 mg/L 6-BA ＋ 0.10 mg/LNAA is the best hormones combination which was obviously good for seedlings＇ rejuvenation, while the groups with 1. 250 times Co2＋ or 150g/L banana juice also performed a positive role on seedlings＇ rejuvenation. |
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| Keywords: | Dendrobium officinate Kimura et Migo tissue culture seedlings medium optimization |
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