| Expression of a begomoviral DNAβ gene in transgenic Nicotiana plants induced abnormal cell division |
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| 作者姓名： | Cui XF Li YQ Hu DW Zhou XP |
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| 作者单位： | InstituteofBiotechnology,ZhejiangUniversiry.Hangzhou310029.China |
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| 摘 要： | An increasing number ofmonopartite begomoviruses are being identified that a satellite molecule (DNAβ) is required to induce typical symptoms in host plants. DNAβ encodes a single gene (termed βCl) encoded in the complementary-sense. We have produced transgenic Nieotiana benthamiana and N. tabaeum plants expressing the βC1 gene of a DNAβ associated with Tomato yellow leaf curl China virus (TYLCCNV), under the control of the Cauliflower mosaic virus 35S promoter. Transgenic plants expressing 13C1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabaeum leaves showed abnormal outgrowths of transgenic N. tabaeum to be due to disorganizedcell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the βC1 protein showed that the 13C 1 protein accumulated in the nuclei of cells. The possible biological function of the βC1 1protein was discussed.
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| 关 键 词： | DNAβ 基因转移 植物 细胞 花烟草 |
Expression of a begomoviral DNAbeta gene in transgenic Nicotiana plants induced abnormal cell division |
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| Cui XF,Li YQ,Hu DW,Zhou XP.Expression of a begomoviral DNAbeta gene in transgenic Nicotiana plants induced abnormal cell division[J].Journal of Zhejiang University Science,2005,6(2):83-86. |
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| Authors: | Cui Xiao-feng Li Yun-qin Hu Dong-wei Zhou Xue-ping |
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| Abstract: | An increasing number of monopartite begomoviruses are being identified that a satellite molecule (DNAbeta) is required to induce typical symptoms in host plants. DNAbeta encodes a single gene (termed betaC1) encoded in the complementary-sense. We have produced transgenic Nicotiana benthamiana and N. tabacum plants expressing the betaC1 gene of a DNAbeta associated with Tomato yellow leaf curl China virus (TYLCCNV), under the control of the Cauliflower mosaic virus 35S promoter. Transgenic plants expressing betaC1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabacum leaves showed abnormal outgrowths of transgenic N. tabacum to be due to disorganized cell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the betaC1 protein showed that the betaC1 protein accumulated in the nuclei of cells. The possible biological function of the betaC1 protein was discussed. |
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