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| 长期递增负荷跑台运动和营养补充对大鼠肾脏EPO水平和mRNA表达的影响 | |
| 引用本文： | 赵杰修,田野,曹建民,金丽,满君,谢敏豪.长期递增负荷跑台运动和营养补充对大鼠肾脏EPO水平和mRNA表达的影响[J].体育科学,2004,24(8):26-29. |
| 作者姓名： | 赵杰修 田野 曹建民 金丽 满君 谢敏豪 |
| 作者单位： | 1. 国家体育总局体育科学研究所,生物研究中心,北京,100061 2. 北京体育大学,运动生化教研室,北京,100084 3. 武汉体育学院,人体运动科学系,湖北,武汉,430079 |
| 基金项目： | 国家自然科学基金资助项目 (3 0 2 70 64 2 )，国家科技部奥运攻关基金资助项目 (2 0 0 2 BA90 4B0 4-4 ) |
| 摘 要： | 为了研究肾脏EPO代谢在运动性贫血发生机理中的功能和作用，及营养补充对运动性贫血防治效果的作用机制，对30只雄性Wistar大鼠进行等量随机分组：对照组(C)、运动组(P)和运动 营养组(G)。11周的跑台运动和营养补充结束后，应用RT—PCR和ELISA方法测试大鼠肾脏EPO基因表达和活性水平。结果发现，肾脏组织匀浆液EPO水平并未表现出对照组、运动 营养组、运动组的组间差异，而对于肾脏EPO mRNA表达指标在运动组分别显著高于对照组和运动 营养组，提示肾脏EPO活性水平可能并非机体运动性贫血的发病因素。 |
| 关 键 词： | 递增负荷 跑台 营养 基因 促红细胞生成素 运动性贫血 动物实验 鼠 |
| 文章编号： | 1000-677X(2004)08-0026-04 |
Effects of Long-term Treadmill Exercise and Nutrition Supplementation on EPO Activity and mRNA Gene Expression of Rat Kidney |
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| ZHAO Jie-xiu,TIAN Ye,CAO Jian-min,JIN Li,MAN Jun,XIE Min-hao Sport Biological Center,China Institute of Sport Science,Beijing ,China, Kinesiology.Effects of Long-term Treadmill Exercise and Nutrition Supplementation on EPO Activity and mRNA Gene Expression of Rat Kidney[J].China Sport Science,2004,24(8):26-29. | |
| Authors: | ZHAO Jie-xiu TIAN Ye CAO Jian-min JIN Li MAN Jun XIE Min-hao Sport Biological Center China Institute of Sport Science Beijing China Kinesiology |
| Institution: | ZHAO Jie-xiu1,TIAN Ye1,CAO Jian-min2,JIN Li3,MAN Jun2,XIE Min-hao21 Sport Biological Center,China Institute of Sport Science,Beijing 100061,China,2 Kinesiology Department of Beijing Sports University,Beijing 100084,China,3 Kinesiology Department of Wuhan Institute of Physical Education,Wuhan 430000,China. |
| Abstract: | In order to investigate the possible role of kidney EPO metabolism in the development of exercise anemia,30 male Wistar rats are randomly assigned to three groups:control(C,n=10),exercise(P,n=10),exercise +nutrition(G,n=10).At the end of eleventh week of treadmill and nutrition supplementation,gene expression and activity of kidney EPO in rats are measured with RT-PCR and ELISA method.The result shows that there is no significant change in EPO activity.However,gene expression level of EPO in P group is higher than that of C and G groups respectively.It suggests that EPO activity of kidney probably is not the mechanism of exercise anemia. |
| Keywords: | increasing load treadmill nutrition gene Erythropoietin exercise anemia animal experiment rat |
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