| 肝素酶的原核表达与表达条件优化 |
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| 引用本文： | 杨嫦娇,郑丽燕,吴文林.肝素酶的原核表达与表达条件优化[J].泉州师范学院学报,2014(2):20-22. |
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| 作者姓名： | 杨嫦娇 郑丽燕 吴文林 |
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| 作者单位： | 泉州师范学院化学与生命科学学院,福建泉州362000 |
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| 基金项目： | 泉州市科技局项目（2010257）;泉州师范学院校大学生科研基金资助项目（2010DKJ06）. |
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| 摘 要： | 利用PCR技术从肝素黄杆菌克隆到肝素酶HepI基因，通过双酶切将其克隆到原核表达载体pGEX-4T-2中，转化大肠杆菌E．coliBL21感受态细胞，获得基因工程重组菌．12℃下IPTG诱导表达12h，Glutathione Sepharose 4B纯化后获得较高纯度的HepI酶蛋白．
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| 关 键 词： | 肝素酶HepI 重组 表达 纯化 |
Prokaryotic Expression and Purification of Heparinases I |
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| YANG Chang-e. ZHEN Li-yan,WU Wen-lin.Prokaryotic Expression and Purification of Heparinases I[J].Journal of Quanzhou Normal College,2014(2):20-22. |
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| Authors: | YANG Chang-e ZHEN Li-yan WU Wen-lin |
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| Institution: | (School of Chemistry and Life Science, Quanzhou Normal University, Fujian 362000,China) |
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| Abstract: | HepariTzases I gene was cloned from F.heparinum by PCR. After digested by Not I and Sma I,it was cloned into pGEX-4T-2 plasmid with the right reading frame sequence to construct the expression vector. This plasmid was used to transfer E.coli BI.21.After inducing with IPTG at 12 ℃ for 12 h,the fusion protein was purified with Glutathione Sepharose 4B. |
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| Keywords: | Heparinases I recombination expression purification |
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