| rDNA片段的序列分析在苦苣苔亚科系统学研究中的应用 |
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| 引用本文： | 汪小全,李振宇.rDNA片段的序列分析在苦苣苔亚科系统学研究中的应用[J].中国科学院研究生院学报,1998,36(2):97-105. |
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| 作者姓名： | 汪小全 李振宇 |
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| 摘 要： | 本文测定了苦苣苔亚科4族、5属、5种植物的核糖体DNA中的内转录间隔区(ITS)序列及5．8s
rRNA基因的3′端序列。这几种苦苣苔亚科植物的ITS-1的长度范围为234～258 bp，ITS-2的长度范
围为218～246bp。Whytockia bijieensis的ITS-1(258bP)和ITS-2(218 bp)在长度、序列及GC含量上
均与其它几个种有较大差异，其代表的尖舌苣苔族可能很早就自苦苣苔亚科的祖先沿单独的一个分支
演化。以w．bijieensis作为功能性外类群，运用PAUP软件分析仅得到一个最简约树。在简约树上，
Cyrtandra umbelliferm、Briggsia longipes和Anna mollifolia形成一个单系群，bootstrap分析对该分支的
支持强度达97％，Chirita crasslfolia位于该分支的基部。由于系统树上Cyrtandra umbellifera代表的
浆果苣苔族和Anna mollifolid代表的芒毛苣苔族均起源于长蒴苣苔族，结合这3个族在形态上存在过渡系列，建议将浆果苣苔族和芒毛苣苔族均并入长蒴苣苔族。
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| 关 键 词： | 苦苣苔亚科 ITS 分子系统发育 |
The Application of Sequence Analysis of rDNA Fragment to the Systematic Study of the Subfamily Cyrtandroideae (Gesneriaceae) |
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| WANG Xiao-Quan,LI Zhen-Yu.The Application of Sequence Analysis of rDNA Fragment to the Systematic Study of the Subfamily Cyrtandroideae (Gesneriaceae)[J].Journal of the Graduate School of the Chinese Academy of Sciences,1998,36(2):97-105. |
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| Authors: | WANG Xiao-Quan LI Zhen-Yu |
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| Abstract: | A molecular phylogeny of the subfamily Cyrtandroideae represented by five genera
of four tribes was constructed using sequence analysis of the internal transcribed spacers
(ITS) and partial 5.8 S rRNA gene (3’end) of nuclear ribosomal DNA. Direct PCR
sequencing method was used in the study. The sequences of ITS-1 in the five species range
from 234 bp to 258 bp in size and those of ITS-2 from 218 bp to 246 bp. The ITS-1 (258
bp) and ITS-2 (218 bp) of Whytockia bijieensis differ greatly from those of the other species
in size, sequence and G + C content, and therefore the tribe Klugieae represented by W.
bijieensis may have diverged from the ancestor of the subfamily Cyrtandroideae at a very
early time. In PAUP analysis, W. bijieensis was used as the functional outgroup, and only
one most parsimonious Fitch tree was obtained through exhaustive search. The tree has 353
steps, with CI = 0. 932 and RI = 0. 529. In the tree, Chirita crassifolia is basal to a
monophyletic group comprising Cyrtandra umbellifera, Briggisia longipes and Anna
mollifolia, and the monophyletic group is strongly supported by the bootstrap value (97).
The tribes Trichosporeae and Cyrtandreae represented respectively by Anna mollifolia and
Cyrtandra urnbellifera both evolved from the tribe Didymorcarpeae, which can explain why
many intermediate taxa exist among the three tribes. According to this, the present authors
suggest that the tribe Trichosporeae and the tribe Cyrtandreae be merged with the tribeDidymocarpeae. |
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| Keywords: | Cyrtandroideae Internal transcrlbedspacers(ITS) Molecularphylogeny |
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