| 不同外源激素对绞股蓝伤组织诱导的影响 |
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| 引用本文： | 蔡正旺,刘静.不同外源激素对绞股蓝伤组织诱导的影响[J].安康学院学报,2014(5):108-111. |
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| 作者姓名： | 蔡正旺 刘静 |
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| 作者单位： | 安康学院 农学与生命科学学院,陕西 安康,725000 |
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| 基金项目： | 安康学院高层次人才科研启动项目 |
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| 摘 要： | 以绞股蓝茎尖、茎段、叶片为外植体，研究了不同种类、不同浓度配比的外源激素对绞股蓝愈伤组织诱导的影响。结果表明：茎尖在培养基MS＋6- BA 1.0 mg·L-1＋NAA 0.02 mg·L-1中的诱导效果最好，其诱导率为75.0%；茎段在培养基MS＋6- BA 2.0 mg·L-1＋NAA 0.2 mg·L-1中的诱导效果最好，其诱导率为81.8%；叶片在培养基MS＋6- BA 1.0 mg·L-1＋NAA 0.15 mg·L-1中的诱导效果较好，其诱导率为69.2%。
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| 关 键 词： | 外源激素 绞股蓝 愈伤组织 诱导 影响 |
Effects of Different and Concentration of Exogenous Hormone on Callus Induction of Gynostemma Pentaphyllum |
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| CAI Zhengwang,LIU Jing.Effects of Different and Concentration of Exogenous Hormone on Callus Induction of Gynostemma Pentaphyllum[J].Journal of Ankang Teachers College,2014(5):108-111. |
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| Authors: | CAI Zhengwang LIU Jing |
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| Institution: | (College of Agriculture and Life Science, Ankang University, Ankang 725000, Shaanxi, China) |
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| Abstract: | Gynostemma shoot tips,stems,leaves as explants to MS medium,of different types,different proportions of exogenous hor-mones on callus induction of Gynostemma. The results show that：the concentration of exogenous hormones is different for different shoot tip,tem,and leaf callus induction. In the medium MS＋6- BA 1.0 mg/L＋NAA 0.02mg/L in the shoot tips of the best induction effect, the induction rate of 75.0%, and the callus growth is good;in the medium MS ＋6- BA2.0mg/L＋ NAA 0.2mg/L in stems induced the best,the induction rate was 81.8%,and the callus grew well;n the medium MS＋6- BA 1.0mg/L＋NAA 0.15mg/L leaves induced ef-fect is better,its induction rate of 69.2%. |
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| Keywords: | exogenous hormone Gynostemma pentaphyllum callus induce effect |
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