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丛枝菌根真菌AMF群落物种多样性研究技术进展
引用本文:杨如意,陈欣,唐建军,陈静,蒋琦清,Shui-jin,HU.丛枝菌根真菌AMF群落物种多样性研究技术进展[J].科技通报,2005,21(6):668-673.
作者姓名:杨如意  陈欣  唐建军  陈静  蒋琦清  Shui-jin  HU
作者单位:1. 浙江大学,生命科学学院,杭州,310029
2. 浙江大学,生命科学学院,杭州,310029;美国北卡罗莱纳州立大学,植物病理系,Raleigh27695-7616
3. 美国北卡罗莱纳州立大学,植物病理系,Raleigh27695-7616
基金项目:国家自然科学基金资助项目(39870149)及国家杰出青年科学基金海外合作项目(30228005)
摘    要:作为一类具有重要生态学意义的微生物类群,丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)的研究很早就引起人们的重视。但是,传统的研究方法不但耗时,费力,精确度低,而且难以大批量地对不同群落进行比较研究。近年来,基于PCR的分子标记技术的应用彻底改变了这一状况。本文综述了多种AMF群落及物种多样性的研究方法,并对传统方法和分子标记技术的优缺点做了比较,着重介绍了分子标记在这一领域的最新应用成果,对该方法存在的问题和应用前景做了总结和展望。

关 键 词:丛枝菌根真菌  群落结构  物种多样性  分子标记
文章编号:1001-7119(2005)06-0668-06
收稿时间:2004-06-04
修稿时间:2004年6月4日

Methods Employed in Species Diversity Determination of Arbuscular Mycorrhizal Fungi (AMF) Colonized in Terrestrial Ecosystem
YANG Ru-yi,CHEN Xin,TANG Jian-jun,CHEN Jing,JIANG Qi-qing,Shui-jin,HU.Methods Employed in Species Diversity Determination of Arbuscular Mycorrhizal Fungi (AMF) Colonized in Terrestrial Ecosystem[J].Bulletin of Science and Technology,2005,21(6):668-673.
Authors:YANG Ru-yi  CHEN Xin  TANG Jian-jun  CHEN Jing  JIANG Qi-qing  Shui-jin  HU
Institution:1. Department of Biology, College of Life Sciences, Zhejiang University, Hangzhou 310029, China; 2. Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695-7616, USA
Abstract:As a kind of microorganism with ecological importance,arbuscular myco rrhizal fungi han been attracting effortof researchers very early.However,trad itional methods were time consuming,labourious,imprecise and furthermore couldn't be compared among different communities on a large scale.Newly appeared mol ecular marker techniques based on PCR changed this situation thoroughly.This ar ticle particularized several researching methods on community and biodiversity of AMF and compared the strongpoints and shortcomings between traditional methods and molecular marker techniques,emphasized the new production of molecular marker in this field,summarized the existing problems and prospected its application foreground.
Keywords:arbuscular mycorrhizal fungi  community structure  species diversity  molecular marker
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