A rabbit model for studying hypocholesterolemic effect of drugs and hypocholesterolemic effect of extracts of garlic (Allium sativum) |
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| Authors: | P Ratnakar P S Murthy |
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| Institution: | (1) Department of Biochemistry, University College of Medical Sciences and GTB Hospital, Shahdara, 110095 Delhi;(2) Indian Journal of Clinical Biochemistry, B164, Sector 14, 201301 Noida, U.P.;(3) Childrens Hospital, University of Pennsylvania, Philadelphia, PA, U.S.A. |
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| Abstract: | An experimental model of hypercholesterolemic rabbits suitable for studying the hypocholesterolemic effect of compounds was
developed. Rabbits were made hypercholesterolemic by oral administration of cholesterol (100mg/kg body weight/day) suspended
in groud nut oil by gastric intubation (Ryle's tube). Cholesterol can be given to rabbits from 10 days to 6 months depending
on the degree of hypercholesterolemia required and duration of study of hypocholesterolemic effect. In one month cholesterol
feeding experiment, the serum cholesterol level in normal controls (not given cholesterol) was 67±11.3 mg/dl and in cholesterol
fed animals 191.3±70.6 mg/dl. In 2 months experiment, besides hypercholesterolemia, abnormal serum lipid profile and increase
in lipid content in liver, heart and aorta were also seen. To such animals when water and methanol extracts of garlic were
given along with cholesterol, there was significant decrease in serum cholesterol level. The advantage of this method over
the method in which cholesterol is mixed with diet to induce hypercholesterolemia is that exactly same and calculated amount
of cholesterol can be given for each animal. This minimises the variations in serum cholesterol levels in different animals. |
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| Keywords: | Animal model Hypercholesterolemia Hypocholesterolemic effect garlic Allium sativum |
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