| 寡聚糖激发子诱导杨树抗溃疡病的生化机制 |
| |
| 引用本文： | 李堆淑.寡聚糖激发子诱导杨树抗溃疡病的生化机制[J].商洛学院学报,2011,25(6):38-42. |
| |
| 作者姓名： | 李堆淑 |
| |
| 作者单位： | 商洛学院生物医药工程系,陕西商洛,726000 |
| |
| 基金项目： | 国家“十五”科技攻关项目(2001BA501A09); 陕西省教育厅科研计划项目(2010JK522); 陕西省商洛市2010年科研计划项目(SK12010-10) |
| |
| 摘 要： | 用已筛选的3种寡聚糖激发子A1、B、C3分别诱导毛白杨愈伤组织,以加无菌水为对照,并在诱导48 h后挑战接种杨树溃疡病菌,测定毛白杨愈伤组织中苯丙氨酸解氨酶、几丁质酶和抗坏血酸氧化酶活性的变化。结果表明,3种寡聚糖激发子A1、B、C3诱导48 h后接种杨树溃疡病菌,毛白杨愈伤组织中苯丙氨酸解氨酶和几丁质酶活性均呈单峰曲线变化,并且变化趋势基本上满足:A1>B>C3>CK(对照接种),而抗坏血酸氧化酶活性的变化为:在发病初期和中期(0-48 h)诱导接种毛白杨愈伤组织均高于对照接种,而在发病后期(t>48 h)对照接种毛白杨愈伤组织高于诱导接种,因此,可以得出寡聚糖激发子A1的诱导抗病性比寡聚糖激发子B和C3都强。
|
| 关 键 词： | 寡聚糖 激发子 杨树溃疡病 毛白杨愈伤组织 诱导抗病性 |
The Biochemical Mechanism of Induced Poplar Resistance to Dothiorella gregaria by Oligosaccharides Elicitors |
| |
| LI Dui-shu.The Biochemical Mechanism of Induced Poplar Resistance to Dothiorella gregaria by Oligosaccharides Elicitors[J].Journal of Shangluo University,2011,25(6):38-42. |
| |
| Authors: | LI Dui-shu |
| |
| Institution: | LI Dui-shu(Biopharmaceutical Engineering Department of Shangluo University,Shangluo,Shaanxi 726000) |
| |
| Abstract: | The Populus tomentosa callus were induced by three oligosaccharides elicitors including elicitor A1,B and C3 selected,with the control of germfree water(CK).First the poplar tomentosa callus were induced by oligosaccharides elicitors for 48 hours,then D.gregaria was challengely inoculated to the inducted and CK callus.The enzyme activities of PAL,Chitinase and ascorbic acid oxidase(APX) of poplar callus were assayed.The results showed that the enzyme activities changes of PAL and Chitinase in poplar callus ... |
| |
| Keywords: | oligosaccharides elicitors Dothiorella gregaria Populus tomentosa callus indution resistance |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
