| 高强度间歇训练对大鼠骨骼肌蛋白合成代谢和分解代谢的影响 |
| |
| 引用本文： | 崔新雯,张一民,汪赞,孔振兴,苏浩,于晶晶.高强度间歇训练对大鼠骨骼肌蛋白合成代谢和分解代谢的影响[J].武汉体育学院学报,2019,53(4):94-100. |
| |
| 作者姓名： | 崔新雯 张一民 汪赞 孔振兴 苏浩 于晶晶 |
| |
| 作者单位： | 1. 国家体育总局体育科学研究所, 北京, 100061;2. 北京体育大学 运动与体质健康教育部重点实验室, 北京, 100084 |
| |
| 基金项目： | 国家"十二五"科技支撑计划课题"运动促进体质健康关键技术的研究与应用"（2012BAK21B00）；中央高校基本科研业务费专项资金资助课题"老年性骨骼肌退变的运动干预机制与效果评价研究"（2015ZD007）。 |
| |
| 摘 要： | 目的:探索连续12周的高强度间歇训练(HIIT)对中年大鼠骨骼肌蛋白合成和蛋白分解的影响。方法:16只9月龄Wistar大鼠随机分安静组(C组)和HIIT组(H组)。C组大鼠正常饮食生活,无训练,H组大鼠进行高(80%VO2max)、低强度(40%VO2max)的间歇运动,每周训练5次共12周。干预结束后取趾长伸肌,称湿重,Western Blot检测趾长伸肌蛋白合成相关蛋白、泛素蛋白酶体关键组分、细胞自噬相关蛋白的表达,电镜观察自噬体结构。结果:相比C组:HIIT提高了趾长伸肌湿重;提高了Akt^( Ser473)和4E-BP1Thr37/46蛋白水平,但对mTORSer2448和P70 S6KThr389无明显影响;降低了MuRF-1和MAFbx的蛋白表达,增加了自噬体,且提高了LC3II/LC3I和PGC-1α蛋白表达,降低了P62蛋白含量,但对LC3II、ULK1Ser757磷酸化水平、Beclin-1的蛋白表达无明显影响。结论:HIIT有利于中年大鼠骨骼肌质量的提高,可能是以下途径共同作用的结果:促进蛋白合成,调节泛素-蛋白酶体系统分解途径的适宜活性,提高基础自噬水平和自噬流的通畅性。
|
| 关 键 词： | 运动生理学 高强度间歇训练 蛋白质代谢 骨骼肌蛋白合成 蛋白分解 细胞自噬 |
| 收稿时间： | 2019-01-23 |
Protein Synthesis and Degradation in Skeletal Muscles of Rats Following High Intensity Interval Training |
| |
| CUI Xinwen,ZHANG Yiming,WANG Zan,et al.Protein Synthesis and Degradation in Skeletal Muscles of Rats Following High Intensity Interval Training[J].Journal of Wuhan Institute of Physical Education,2019,53(4):94-100. |
| |
| Authors: | CUI Xinwen ZHANG Yiming WANG Zan |
| |
| Institution: | 1. China Inst. of Sport Science, Beijing 100061, China;2. Beijing Sport Univ., Beijing 100084, China |
| |
| Abstract: | The aim of this study was to investigate the changes of protein synthesis and degradation in skeletal muscles of rats following high intensity interval training (HⅡT). Nine months old male Wistar rats were divided in control group (C group) and high intensity interval training (HⅡT) group (H group). The weight of extensor digitorum longus(EDL), autophagosome and the expression of protein synthesis related proteins, ubiquitinated protein, MuRF-1, MAFbx, autophagy-related proteins in EDL were assayed 12 week after the HⅡT training. Compared to the C group, HⅡT effectively improved the weight of EDL, the expression of phosphorylated protein of Akt Ser473 and 4E-BP1Thr37/46, but showed no impact on the expression of mTORSer2448 and P70 S6KThr389. H group decreased the expression ofMuRF-1and MAFbx. HⅡT increased the autophagosome, improved the LC3Ⅱ/LC3I and PGC-1α, and decreased the level of P62, but showed no impact on the expression ofLC3Ⅱ, ULK1Ser757 and Beclin-1. It could be conclusion that HⅡT could enhance the skeletal muscle mass,which might be benefit from promoting the protein synthsis, adjusting the appropriate activation level of the ubiquitin-proteasome pathway, improving the basic autophagic activity and autophagy flow. |
| |
| Keywords: | high intensity interval training protein metabolism protein synthesis protein degradation autophagy |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《武汉体育学院学报》浏览原始摘要信息 |
| 点击此处可从《武汉体育学院学报》下载免费的PDF全文 |
|
