| 多聚半乳糖醛酸酶抑制蛋白抗血清的制备及鉴定 |
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| 引用本文： | 阮期平,赵莉,周立,李建吾,郑远棋.多聚半乳糖醛酸酶抑制蛋白抗血清的制备及鉴定[J].绵阳师范学院学报,1997(Z1). |
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| 作者姓名： | 阮期平 赵莉 周立 李建吾 郑远棋 |
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| 作者单位： | 绵阳师范高等专科学校 绵阳621000(阮期平，赵莉)，四川大学生物系 成都610064(周立，李建吾，郑远棋) |
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| 摘 要： | 用电泳纯的小麦多聚半乳糖醛酸酶抑制蛋白免疫新西兰雄兔,获得了特异性强,效价较高的小麦.多聚半乳糖醛酸酶抑制蛋白抗血清.用此抗血清采用Dot—immunobinding assay,对流免疫电泳二种方法测定PGIP的检出限量,由此建立了多聚半糖醛酸酶抑制蛋白的Dot—immunobinding检测方法,并对制备该抗血清的意义予以讨论.
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| 关 键 词： | 小麦多聚半乳糖醛酸酶抑制蛋白 Dot-immunobindingassay 抗血清 对流免疫电泳 免疫双扩散 |
Preparation and Identification of the Antisera Against the Polygalacturonase Inhibiting Protein in Wheat |
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| Ruan Qiping,Zhao Li.Preparation and Identification of the Antisera Against the Polygalacturonase Inhibiting Protein in Wheat[J].Journal of Mianyang Normal University,1997(Z1). |
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| Authors: | Ruan Qiping Zhao Li |
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| Abstract: | Specific polyclonal antisera against polygalacturonase inhibiting protein (PGIP) in wheat have been obtained. The specificity and crossreaction of antibodies were determined in dou-ble immunodiffusion. The titer of antisera was determined by using double immunodiffusion. 1. 95 ?10~(-9)mg PGIP and 3. 125 ?10~(-4)mg PGIP could be examined by dot immunobinding assay and countercurrent immunoelectrophoresis. The dot immunobinding assay of PGIP in plant tis-sues has been built. The significance of antisera against PGIP was discussed. |
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| Keywords: | Polygalacturonase inhibiting protein dot immunobinding assay antiserum countercurrent immunoelectrophoresis double immunodiffusion |
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