Epigallocatechin-3-gallate affects the growth of LNCaP cells via membrane fluidity and distribution of cellular zinc |
| |
| Authors: | Jun-guo Yang Hai-ning Yu Shi-li Sun Lan-cui Zhang Guo-qing He Undurti N Das Hui Ruan Sheng-rong Shen |
| |
| Institution: | (1) Department of Food Science and Nutrition, Zhejiang University, Hangzhou, 310029, China;(2) College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, 310032, China;(3) Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China;(4) UND Life Sciences, 13800 Fairhill Road, #321, Shaker Heights, OH 44120, USA |
| |
| Abstract: | Objective: To evaluate effects of epigallocatechin-3-gallate (EGCG) on the viability, membrane properties, and zinc distribution, with and without the presence of Zn2+, in human prostate carcinoma LNCaP cells. Methods: We examined changes in cellular morphology and membrane fluidity of LNCaP cells, distribution of cellular zinc, and the incorporated portion of EGCG after treatments with EGCG, Zn2+, and EGCG+Zn2+. Results: We observed an alteration in cellular morphology and a decrease in membrane fluidity of LNCaP cells after treatment with EGCG or Zn2+. The proportion of EGCG incorporated into liposomes treated with the mixture of EGCG and Zn2+ at the ratio of 1:l was 90.57%, which was significantly higher than that treated with EGCG alone (30.33%). Electron spin resonance (ESR) studies and determination of fatty acids showed that the effects of EGCG on the membrane fluidity of LNCaP were decreased by Zn2+. EGCG accelerated the accumulation of zinc in the mitochondria and cytosol as observed by atomic absorption spectrometer. Conclusion: These results show that EGCG interacted with cell membrane,decreased the membrane fluidity of LNCaP cells, and accelerated zinc accumulation in the mitochondria and cytosol, which could be the mechanism by which EGCG inhibits proliferation of LNCaP cells. In addition, high concentrations of Zn2+ could attenuate the actions elicited by EGCG. |
| |
| Keywords: | Epigallocatechin-3-gallate (EGCG) Zinc LNCaP Membrane fluidity |
| 本文献已被 万方数据 SpringerLink 等数据库收录！ |
|
