Use of Computer Modeling of Site-Directed Mutagenesis of a Selected Enzyme: A Class Activity for an Introductory Biochemistry Course |
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| Authors: | Jones Marjorie A Shoffner Raymond Friesen Jon |
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| Institution: | (1) Department of Chemistry, Illinois State University, Normal, Illinois, 61790-4160 |
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| Abstract: | For an introductory undergraduate biochemistry class, we developed an interactive, out of classroom, team project that would allow students to gain a better understanding of protein structure and function. This project involved the use of the molecular visualization program RasMol and computer simulated site-directed mutagenesis of selected amino acids of the enzyme, Uroporphyrinogen decarboxylase. Survey data from students after completion of the project indicated that they had developed a more complete understanding of protein three-dimensional structure leading to functionality. |
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| Keywords: | site-directed mutagenesis computer modeling enzyme structure and functional relationships |
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