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接骨草的绿原酸含量测定及方法学考察
引用本文:姚元枝,李爱民,全妙华,汤立志.接骨草的绿原酸含量测定及方法学考察[J].怀化师专学报,2013(11):9-11.
作者姓名:姚元枝  李爱民  全妙华  汤立志
作者单位:[1]怀化学院生命科学系,湖南怀化418008 [2]怀化学院民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418008 [3]怀化学院湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化418008
基金项目:湖南省科技计划项目(2011NK3045);民族药用植物资源研究与利用湖南省重点实验室开放基金项目(ZDSYSJJ2013-2);湖南省教育厅科学研究项目(10C1062).
摘    要:采取紫外分光光度法测定接骨草的绿原酸含量,为接骨草合理利用提供实验依据.结果表明,接骨草全草及不同部位的绿原酸含量由高到低依次为:花、叶、果、全草、茎和根状茎,并且差异均达到极显著水平(P〈0.01).精密度实验、重复性实验、稳定性实验、加样回收实验等方法学考察表明,紫外分光光度法测定接骨草绿原酸含量效果好,稳定性强,方法可靠.

关 键 词:接骨草  不同部位  绿原酸  含量

Determination of Contents of Chlorogenic Acid in Sambucus chinensis Lindl. and Methodology Investigation
YAO Yuan-zhi,QUAN Miao-hua,TANG Li-zhi.Determination of Contents of Chlorogenic Acid in Sambucus chinensis Lindl. and Methodology Investigation[J].Journal of Huaihua Teachers College,2013(11):9-11.
Authors:YAO Yuan-zhi  QUAN Miao-hua  TANG Li-zhi
Institution:1. Department of Life Sciences ; 2. Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources ; 3. Key Laboratory of Hunan Higher Education for Xiangxi Medicinal Plant and Ethnobotany , Huaihua University, Huaihua, Hunan 418008)
Abstract:The contents of chlorogenic acid in Sambucus chinensis Lindl. were studied by UV spectrophotometry, in order to provide the experimental basis for the rational uses of Sambucus chinensis Lindl. The results showed that the contents of chlorogenic acid in whole plants and different parts of Sambucus chinensis Lindl. in order were flowers, leaves, fruits, whole plants, stems, rhizomes, and differences were highly significant (p 〈 O. 01). The resuhs of precision, repeat, stability and standard recovery experiments showed that the effects of the determination of the contents of chlorogenic acid in Sambucus chinensis Lindl. by UV spectrophotometry were very well with good stabilities, and the mothod was credible and easy to use.
Keywords:Sambucus chinensis Lindl    different parts  chlorogenic acid  content
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