| D6S1043基因座检出三带型等位基因2例及遗传分析 |
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| 引用本文： | 王芳,胡拓,石君,赵熙.D6S1043基因座检出三带型等位基因2例及遗传分析[J].大众科技,2016(7):87-88. |
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| 作者姓名： | 王芳 胡拓 石君 赵熙 |
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| 作者单位： | 1. 常德市公安局刑事科学技术研究所，湖南 常德 415000; 常德市广德司法鉴定中心，湖南 常德 415000;2. 常德市公安局刑事科学技术研究所,湖南 常德,415000 |
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| 摘 要： | 目的:在亲子鉴定案件中检出D6S1043基因座三带型。方法:提取个体不同组织DNA样本,经复合荧光PCR扩增和毛细管电泳分析2716个案例(共6246名个体)的STR分型。结果:在1个父子单亲鉴定案例中,2名个体的D6S1043基因座上出现三带型等位基因。用Power Plex?21荧光标记复合扩增试剂盒对包含D6S1043在内的21个STR基因座进行检测,父亲D6S1043基因座分型为17/18/21,儿子分型为10/17/18,电泳峰高和峰面积之比均为1∶1∶1,用SinofilerTM试剂盒复核检测结果相同。结论:在本文发现的案例中,父亲的等位基因17和18均遗传给了儿子,导致儿子表现为三带型等位基因。D6S1043三带型非常罕见,在分析时应采用不同方法或者试剂盒进行验证,保证鉴定结果的可靠性。
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| 关 键 词： | 短串联重复序列 D6S1043基因座 三带型等位基因 |
Genetic analysis of two three-banded allele patterns in D6S1043 locus |
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| Abstract: | Aim:To report a three-banded allele pattern of the STR locus D6S1043 in a paternity identification. Methods:DNA was extracted from different tissue samples of the individual. STR genotype of 2716 cases (6246 individuals) were determined and analyzed by multiplex fluorescent PCR amplication and capillary electrophoresis. Results:Two cases were confirmed to have three-banded allele locating in D6S1043 locus in a father-child case. PowerPlex?21 multiplex fluorescent PCR amplication system detected 21 STR loci including D6S1043 locus.Father's alleles in D6S1043 locus were17/18/21.Child’s alleles in D6S1043 locus were10/17/18, the height and area ratio in D6S1043 locus both were about 1:1:1. The result of three-banded allele of D6S1043 locus was the same as SinofilerTM kit. Conclusions:In this case father's 17 and 18 alleles passed on to his son leading to the three-banded allele pattern. The three-banded allele pattern of the STR locus D6S1043 is rare. To ensure the reliability of analysis results, different methods should be adopted for validation. |
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| Keywords: | STR D6S1043 locus three-banded allele |
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