Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids |
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| Authors: | Zhang Guo-liang Wu Yue-ting Qian Xin-ping and Meng Qin |
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| Institution: | (1) School of Materials Science and Chemical Engineering, Zhejiang University, 310027 Hangzhou, China;(2) Institute of Biological and Environmental Engineering, Zhejiang University of Technology, 310032 Hangzhou, China |
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| Abstract: | The potential biodegradation of crude oil was assessed based on the development of a fermentative process with a strain ofPseudomonas aeruginosa which produced 15.4 g/L rhamnolipids when cultured in a basal mineral medium using glycerol as a sole carbon source. However,
neither cell growth nor rhamnolipid production was observed in the comparative culture system using crude oil as the sole
carbon source instead. As rhamnolipid, an effective biosurfactant, has been reported to stimulate the biodegradation of hydrocarbons,
1 g/L glycerol or 0.22 g/L rhamnolipid was initially added into the medium to facilitate the biodegradation of crude oil.
In both situations, more than 58% of crude oil was degraded and further converted into accumulated cell biomass and rhamnolipids.
These results suggest thatPseudomonas aeruginosa could degrade most of crude oil with direct or indirect addition of rhamnolipid. And this conclusion was further supported
by another adsorption experiment, where the adsorption capacity of crude oil by killed cell biomass was negligible in comparison
with the biologic activities of live cell biomass.
Project supported by Science Foundation from China Petroleum and Chemical Corporation, China |
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| Keywords: | Rhamnolipid Crude oil Biodegradation Pseudomonas aeruginosa |
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