| A rapid and accurate 3-（4,5-dimethyl thiazol-2-yl）-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example |
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| 作者姓名： | Wang F Cao LT Hu SH |
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| 作者单位： | Department of Veterinary Medicine,College of Animal Sciences,Zhejiang University,Hangzhou 310029,China |
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| 基金项目： | Project （Nos. 2005C22035 and 2005C12015） supported by the Department of Science and Technology of Zhejiang Province, China |
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| 摘 要： | The objective of this study is to propose a more accurate and faster MTT 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r2=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125～32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7～8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24～28 h for ADA. The MCA provides an accurate and rapid method for quantifi-cation of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.
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| 关 键 词： | MMT 比色测定 乳酸链球菌肽 微球菌 四唑 溴化物 |
| 收稿时间： | 13 December 2006 |
| 修稿时间： | 2006-12-132007-05-09 |
A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example |
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| Wang F,Cao LT,Hu SH.A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example[J].Journal of Zhejiang University Science,2007,8(8):549-554. |
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| Authors: | Wang Fang Cao Li-ting Hu Song-hua |
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| Institution: | (1) Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310029, China |
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| Abstract: | The objective of this study is to propose a more accurate and faster MTT 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium
bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example.
After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number
of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then
MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r
2=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125∼32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only
7∼8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24∼28 h for ADA. The MCA provides
an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other
antimicrobial substances.
Project (Nos. 2005C22035 and 2005C12015) supported by the Department of Science and Technology of Zhejiang Province, China |
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| Keywords: | MTT colorimetric assay Nisin Micrococcus luteus |
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