| 导入粟DNA的小麦变异系酯酶同工酶分析 |
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| 引用本文： | 梁顺祥.导入粟DNA的小麦变异系酯酶同工酶分析[J].青海科技,2000,7(3):11-13. |
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| 作者姓名： | 梁顺祥 |
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| 作者单位： | 青海大学农学系!西宁810016 |
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| 基金项目： | 青海省科技厅科技计划项目 |
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| 摘 要： | 彩和ＰＡＧＥ方法，对从导入珍珠粟ＤＮＡ的两个春小麦受体（佛约２８和青春４４２５）中所选的１７个变异系进行了酯酶同工酶分析，结果表明，变异系与受体的酯酶同工酶图谱基本相同；同时，在变异系中出现了供体酶带和新酶带，而且在酶活性和酶带数等方面也表现出不同程度的变异。说明酯酶同工酶的变异与粟ＤＮＡ导入有密切关系，变异系是外源ＤＮＡ导入的结果，进上步说明花粉管通道法导入外源ＤＮＡ技术是改良小麦品种、丰富小麦
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| 关 键 词： | 外源DNA导入 春小麦 变异系 酯酶同工酶 育种 |
Esterase isoenzyme analysis of spring wheat after introducing millet DNA |
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| LIANG Shun,xiang.Esterase isoenzyme analysis of spring wheat after introducing millet DNA[J].Qinghai Science and Technology,2000,7(3):11-13. |
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| Authors: | LIANG Shun xiang |
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| Abstract: | Millet DAN was introduced into two spring wheat varieties(Huyue 28 and Qingchun 4425)by the pollen tube pathway method.Seventeen variants from their descendents,the two teceptors and the donor were used as the test materials for esterase isoenzyme analysis.It was found that the variants were very similar to the receptors in esterase electrophoresis(PAGE)bands.Several kinds of variations were obviously shown.It was confirmed that the 17 variants are all the transformed strains of millet DNA.It was indicated that the pollen tube pathway technique for exogenous DNA transformation is an effective way to enrich wheat gene pool and improve wheat. |
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| Keywords: | Exogenous DNA introduction Spring wheat Variants Esterase isoenzyme |
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